Although, the knowledge management (KM)-culture research has helped to validate the importance of cultural values for companies' KM initiatives and provided insights into some important values, it still lacks frameworks and analysis outlining how specific types of cultural values might relate to Knowledge Management system (KMS) adoption and subsequent outcomes. In this paper, we provide a three-dimensional framework to help managers articulate how culture affects their unit's ability to create, transfer, and apply knowledge through KMS use. To illustrate the application of the framework, we also present an exploratory case study we have performed in an international organization in the area of development assistance and capacity development.