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1 results

  • An Improved Cryogen for Plunge Freezing

  • William F. Tivol, Ariane Briegel, Grant J. Jensen
  • Journal:
    Microscopy and Microanalysis / Volume 14 / Issue 5 / October 2008
    Published online by Cambridge University Press:
    16 September 2008, pp. 375-379
    Print publication:
    October 2008

  • The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.