Sustainable ruminant production systems depend on the ability of livestock to utilize increased quantities of grazed herbage. The current study aimed to compare the effect of white clover (WC) inclusion and perennial ryegrass (PRG) ploidy on herbage dry matter (DM) production, plant morphology, nutritive value and biological nitrogen (N) fixation (BNF) under high N fertilizer use (250 kg N/ha) and high stocking rates (2.75 livestock units/ha). Four sward treatments (diploid-only, tetraploid-only, diploid-WC, tetraploid-WC) were evaluated over a full grazing season at a farmlet scale. White clover inclusion had a significant effect on herbage DM production, herbage growth rate, tiller density, organic matter digestibility, crude protein and BNF. Tetraploid swards had a lower tiller density, lower sward WC content and post-grazing sward height and increased organic matter digestibility and crude protein than diploid swards. White clover inclusion improved herbage DM production and nutritive value across a full grazing season, with tetraploid and diploid swards producing similar herbage DM yields across the year. Perennial ryegrass ploidy had an effect on WC morphology as plants in diploid-WC swards had narrower, longer stolons, fewer branches and more petioles than tetraploid-WC swards. The current study highlights the benefit of including WC in grass-based systems under a high N fertilizer regime and high stocking rate.