A tandem mass spectrometric procedure was developed to detect sesbanimide A and its isomers in plant materials. Analysis of seed extracts from 10 Sesbania species using this procedure readily detected sesbanimides in Drummond rattlebush, rattlebush, and bagpod sesbania. The toxic sesbanimides were not detected in hemp sesbania or in other species with no known history of animal toxicity.

Fumonisin B1 (FB1) sprayed on intact jimsonweed plants at 10 to 200 μg ml–1 caused chlorosis and necrosis on three- to four-leaf plants and reduced their height and biomass. Excised leaves of jimsonweed were necrotic on both surfaces after treatment with FB1 at 10 to 200 μg ml–1. The degree of damage increased with the concentration of FB1. The minimum amount of FB1 that damaged excised leaves was 0.05 μg. The symptoms of toxicity on sensitive plants varied with concentration of FB1. Symptoms observed included: chlorosis (creeping cucumber), necrosis (jimsonweed and common sunflower), black leaf lesions (sicklepod), curl of tissues (soybean), stunting, defoliation (hemp sesbania and northern jointvetch), and death (prickly sida, spurred anoda, northern jointvetch, and jimsonweed). Monocots (barley, bermudagrass, corn, johnsongrass, rice, sorghum, and wheat) were not visibly affected by FB1. This is the first evidence of the phytotoxic properties of fumonisin B1 against a wide variety of weeds and crop cultivars.

Field studies evaluated response of soybean to Rhizoctonia foliar blight (RFB) disease in combination with varying densities of common cocklebur, hemp sesbania, or johnsongrass. Soybean plants at both V10 and R1 growth stages were not inoculated or inoculated with suspensions containing equal concentrations of Rhizoctonia solani AG-1 IA and IB mycelia. Intensity of RFB was rated weekly beginning at V1 soybean growth stage, and data were used to determine area under disease progress curves. Intensity of RFB was greater in 1993 than in 1994. When averaged across weed species and weed densities, soybean yield in 1993 was reduced 18% in plots inoculated with R. solani compared with those not inoculated. Intensity of RFB, however, did not differ between inoculated and noninoculated plots in 1994. Interactions between R. solani and weed density for RFB intensity and yield were not significant either year. Soybean yields in 1994, however, were reduced by hemp sesbania and johnsongrass in inoculated plots. Soybean maturity was delayed both years when hemp sesbania was present.

Field experiments were established in 1989 and 1990 on silty clay and sandy loam soils to evaluate selective herbicides in combination with non-selective weed control measures in conventional and stale seedbed soybean production. Metribuzin PRE followed by chlorimuron POST controlled sicklepod better with paraquat than with glyphosate. A POST application of imazaquin increased sicklepod and pitted morningglory control by imazaquin PRE alone in a stale seedbed or tillage program. Pitted morningglory control with imazaquin PRE was lower with tillage than with glyphosate or paraquat combinations in a stale seedbed program. All metribuzin plus chlorimuron PRE treatments, whether conventional tillage or stale seedbed, controlled pitted morningglory more than 75%. Hemp sesbania control was above 80% with all metribuzin followed by chlorimuron or metribuzin plus chlorimuron PRE combinations, and less than 70% with all treatments containing imazaquin. Selective herbicides increased yield in stale seedbed when glyphosate or paraquat was added. Imazaquin PRE, imazaquin PRE followed by imazaquin POST, and metribuzin PRE followed by chlorimuron POST tank mixed with glyphosate or paraquat in a stale seedbed program increased yield compared with the same treatments used with tillage.

Field experiments were conducted in 1989 and 1990 on silty clay and sandy loam soils to evaluate weed control and soybean yield with early-April preplant incorporation of selective herbicides in stale seedbed soybean followed by non-selective weed control measures at planting. Metribuzin applied PPI early followed by chlorimuron POST coupled with either glyphosate or paraquat PRE controlled sicklepod, pitted morningglory, and hemp sesbania to the same extent of that treatment applied PPI at planting. All stale seedbed treatments with POST applications and glyphosate, paraquat, or tillage at planting controlled pitted morningglory over 70%. However, imazaquin or metribuzin applied PPI early without a POST treatment controlled sicklepod and pitted morningglory poorly. Frequently, applying PPI herbicides at planting increased control compared with early PPI applications, but this was overcome by POST treatments. Early stale seedbed applications of metribuzin did not result in more than 60% control of hemp sesbania, whereas metribuzin applied PPI at planting controlled over 85%. However, metribuzin plus chlorimuron controlled hemp sesbania at least 74%, regardless of application timing or tillage method, whereas no imazaquin treatment achieved over 65% control. All stale seedbed herbicide treatments increased soybean yield compared with the untreated stale seedbed check. Selective herbicide treatments with either non-selective herbicide in a stale seedbed program resulted in equivalent yield to PPI at planting treatments most often, except with metribuzin.

Field studies were conducted at three locations in Louisiana over two years to evaluate mid-season, foliar-applied acifluorfen, fomesafen, and lactofen for hemp sesbania control in soybean. Acifluorfen and fomesafen were applied POST at 30, 40, 50, 60, 70, 140, and 280 g ai ha–1 and lactofen was applied at 60, 80, 110, and 220 g ai ha–1. The data fit a quadratic model and log transformations were made to determine differences between treatments. Hemp sesbania control was highly correlated with herbicide rate for each herbicide. Averaged over rates of application, acifluorfen and fomesafen provided equivalent control of hemp sesbania, which was greater than that achieved with lactofen. The minimum effective rate of acifluorfen or fomesafen for 80 and 100% control of 50- to 60-cm hemp sesbania was 50 and 140 g ha–1, respectively. The minimum effective lactofen rate to provide at least 80% control was 220 g har–1.