The ability of animals to cope with an increasing parasite load, in terms of resilience and resistance, may be affected by both nutrient supply and demand. Here, we hypothesized that host nutrition and growth potential interact and influence the ability of mice to cope with different parasite doses. Mice selected for high (ROH) or low (ROL) body weight were fed a low (40 g/kg; LP) or high (230 g/kg; HP) protein diet and infected with 0, 50, 100, 150, 200 or 250 L3 infective Heligmosomoides bakeri larvae. ROH-LP mice grew less at doses of 150 L3 and above, whilst growth of ROH-HP and of ROL mice was not affected by infection pressure. Total worm burdens reached a plateau at doses of 150L3, whilst ROH mice excreted fewer worm eggs than ROL mice. Serum antibodies increased with infection dose and ROH mice were found to have higher parasite-specific IgG1 titres than ROL mice. In contrast, ROL had higher total IgE titres than ROH mice, only on HP diets. The interaction between host nutrition and growth potential appears to differentially affect resilience and resistance in mice. However, the results support the view that parasitism penalises performance in animals selected for higher growth.

Hemigrapsus crenulatus is an abundant and frequent decapod crustacean inhabiting estuarine environments, where it must tolerate large shifts in salinity. The present study evaluates the effect of salinity (5, 13, 21 and 30 psu) on the adult physiological processes related to the energy balance. The growth potential (SFG) and the respired oxygen:excreted nitrogen ratio were used as indices of stress. Ingestion, excretion and respiration rates showed a significant dependence on salinity, being higher at low salinities. The assimilation efficiency remained constant along the studied salinity gradient. The assimilation and ingestion rates were inversely related with the salinity. Given this scenario, the growth potential remained constant within the studied salinity gradient, as did the oxygen:nitrogen ratio. The results suggest that the increased energy losses at low salinity due to respiration and excretion are compensated by an increment in the ingestion rate, contributing to the success of H. crenulatus in dynamic habitats such as estuaries.

Artificial selection for improved productivity may reduce an animal's ability to cope with pathogens. Here, we used Roslin mice, uniquely divergently selected for high (ROH) and low (ROL) body weight, to assess interactive effects of differing growth potential and protein nutrition on host resilience and resistance. In a 2×2×6 factorial design, ROH and ROL mice were either sham-infected or infected with 250 L3Heligmosomoides bakeri and fed diets with 30, 80, 130, 180, 230 and 280 g crude protein per kg. The infected ROL-30 treatment resulted in clinical disease and was discontinued. In the remaining ROL mice, infection and feeding treatments did not affect growth but infection reduced weight gain in ROH-30, ROH-80 and ROH-130 mice. Although infection resulted in temporarily reduced food intake (anorexia) in both mouse lines, mean food intake over the whole experiment was reduced in ROH mice only. ROH mice excreted more worm eggs and had higher worm burdens, with relatively fewer female worms, than ROL mice. However, these resistance traits were not sensitive to dietary protein. These results support the view that selection for high growth may reduce the ability to cope with pathogens, and that improved protein nutrition may to some extent ameliorate this penalty.

Recent concern about gene flow from transgenic plants to weedy species has attracted much research on the fitness of their hybrids. However, no studies have been reported on the very early effects of the seed size of hybrids compared with parental plants for germination, seedling establishment and plant growth. We produced hybrids between male sterile Brassica napus L. (oilseed rape) and five weedy relatives, including Brassica juncea (L.) Czern, B. nigra (L.) Koch, B. rapa L., Hirschfeldia incana (L.) Lagrèze-Fossat and Raphanus raphanistrum L. The hybrid seeds formed between B. napus and B. rapa varied in size, while all the hybrid seeds formed with the other species were small. In a direct-seeded field experiment, small seeds of both parents and hybrids had a lower frequency of emergence and a lower seedling survival rate than large seeds, and resulted in later flowering with less biomass. However, no difference was recorded in a transplant experiment, indicating that growth in the juvenile period was sensitive to the small seed class in field conditions only. The optimum environmental conditions in the greenhouse probably homogenized the developmental differences observed at the early stage, and thus reduced the variation during subsequent growth in the field. This point has not been, but should be, considered in risk assessment of transgenic plants. The lower seedling establishment of small-seeded hybrids could hamper further gene flow.