The reproductive biology of Psammobatis lentiginosa was studied in San Matías Gulf (Patagonia, south-west Atlantic). A total of 1033 skates was analysed, total length ranged from 17 to 52 cm for females and from 13 to 55 cm for males. Total length–total weights relationships differed between sexes, with TW = 0.005 × TL3.02 for females and TW = 0.005 × TL2.97 for males. The overall sex-ratio was 1:1. In females, the L50% was 41.06 cm, in males the L50% was 41.38 cm. The mean number of yolked oocytes per skate was 4.22 (±2.41). The mean number of yolked oocytes and the length of the female were linearly related. The diameter maximum of yolked oocytes was 24 mm. The species exhibited a continuous reproductive cycle throughout the year, with a peak of egg-case production during autumn that was synchronous with maximum values of the epididymis and uterus indices. This is the first record of a reproductive aggregation for P. lentiginosa in San Matías Gulf, North Patagonia. This finding together with its continuous presence around the year, suggest that this area is the main distribution area of P. lentiginosa in the south-west Atlantic.