Introduction. Cytokinins enhance axillary shoots, which helps
prolific shoot multiplication in vitro. Very few reports are available on
biochemical changes associated with this in vitro proliferation of Citrus.
The present study was undertaken to find out the influence of two widely
used cytokinins on various morphological and biochemical constituents of
in vitro proliferated shoots of Citrus species. Materials and methods.
Shoots of (0.5 to 1.0) cm long from in vitro proliferated shoots of five
Citrus species were cultured on MS medium supplemented with eight
increasing levels of benzylaminopurine (BAP) or kinetin spread from
(0.25 to 2) mg$\cdot$L-1. After 6 weeks of culture, morphogenetic characters
were observed, and shoot samples were crushed with 5 mL of hot ethanol;
the extract collected was used for the estimation of reducing sugars,
total phenols, ortho dihydric (OD) phenol, amino nitrogen and protein
percentages. Results and discussion. For all the morphogenetic characters
studied, except the shoot number, there were significant interactions
between the Citrus species and the different concentrations of BAP and
kinetine. Increased concentration of cytokinins reduced the amount of
total phenol, OD phenol and total protein. Calamondin recorded the lowest
percent of reducing sugar and protein. The maximum amount of reducing
sugars was recorded for C. volkameriana. Supplementation of BAP resulted
in the highest amount of total and OD phenols, whereas kinetin improved
the reducing sugar and amino nitrogen.