Seven-day-old seedlings of 22 rice cultivars with dominant Glh1, Glh2, Glh3, Glh5, Glh6 and Glh7 and recessive glh4 genes were evaluated for resistance to the green leafhopper (GLH), Nephotettix virescens (Distant), in a no-choice seedling test under uniform infestation at four nymphs per seedling. “IR29” (gene not identified) was the resistant check, while “Taichung Native 1” (no resistance gene) was the susceptible check. Maximum reproducibility for resistance score was obtained 11 days after infestation. “Pankhari 203” (Glh1), “Kalimekri 77–5” (IRRI Accessions 6613 and 6622) (Glh1), “ASD7” (Glh2), “DNJ97” and “Arai” (Glh3), “ASD8” (Glh5), “PTB18” (Glh6), and “Laki 65” (IRRI Accession 6622) and “Lasane” (Glh7) showed consistently resistant reaction under the no-choice test. The lines could be used for allelic studies of GLH resistance and for gene pyramiding.