Field experiments were conducted in 1983 and 1984 to measure the interference of wild oat (Avena fatua L. # AVEFA) removed at various stages of development (two to three leaves, two to three tillers, two nodes, and heading), plus treatments with wild oat not removed, and wild oat-free stands on the growth and yield of spring barley (Hordeum vulgare L. ‘Advance’). The final plant density of barley and wild oat was 160 and 170 plants/m2, respectively. Based on aboveground barley biomass and yield, the critical duration of wild oat interference began about the two-node stage and continued until maturity of the barley. Wild oat interference reduced barley biomass, the number of tiller heads/plant, tiller heads/unit area, and tiller grain yield, but not the number or grain yield of the main-stem heads. Wild oat did not affect the soil matric potential or the barley total plant and soil nitrogen contents. However, wild oat did reduce total water and turgor potential in barley at the boot stage of growth, which may have affected tiller head formation.