In order to satisfy the demands of the modern consumer for food of high quality, low in fat but rich in polyunsaturated fatty acids, farmers from the inner regions of both Italy and Greece in recent years have started to orient their activity to the rearing of autochthonous animal populations, as in the case of the bovine Podolian (Italy) and Katerini (Greece). As both of these populations (raised in two different countries, but under a similar free-range production system) were derived from Bos primigenius, it was considered interesting to compare their meat characteristics. For this target, eight animals were used, subdivided into two groups: Podolian group (reared in Italy and slaughtered at 18 months of age) and Katerini group (reared in Greece and slaughtered at 18 months of age). All animals were fed only on local pasture and they were slaughtered in licensed slaughterhouses in their country of birth and they were analysed in the laboratories of the University of Bari in Italy. The results show that the raw meat of Katerini bulls is less red, has higher moisture and is leaner. The fat of this meat is richer in C18:0 and ω3 fatty acids. These data seem to indicate that the meat produced by the breeds studied can satisfy the dietary demands of the modern consumer. As the Katerini breed is threatened with extinction, this study can offer a reason to the local authorities to make a possible future rescue programme, which necessarily passes through a chemical and nutritional characterization of its meat.