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2 results

  • Chapter 7 - Embryo Assisted Hatching for IVF

  • from Section 1 - Starting a New Laboratory and Training Protocols
  • Edited by Markus H. M. Montag, ilabcomm GmbH, St Augustin, Germany, Dean E. Morbeck, Kindbody Inc, New York City
  • Book:
    Principles of IVF Laboratory Practice
    Published online:
    07 August 2023
    Print publication:
    24 August 2023, pp 47-53

  • Summary

    The zona pellucida surrounding human embryos may be breached to facilitate the process of hatching following in vitro culture, either to promote implantation or in preparation for removal of cells for pre-implantation genetic testing (PGT). This technique is referred to as ‘assisted hatching’ (AH). Since publication of the first studies on AH in the 1990s aimed at improving implantation and pregnancy rates, in which a chemical method was used to breach the zona, multiple variations of the technique have been attempted by practitioners. Application of AH prior to fresh embryo transfer in young, good-prognosis patients has been repeatedly shown to be ineffective. The method continues to be successfully applied to fresh embryos for embryo biopsy and outcome data support application in frozen–thawed (vitrified–warmed) embryos and oocytes prior to transfer. Proper training, patient selection and methodology are important to the clinical success of AH as any other micromanipulation technique. Complete removal of the zona pellucida to avoid trapping has been proposed as an alternative to partial breach but studies are scarce.

  • Effect of vitrification technique and assisted hatching on rabbit embryo developmental rate

  • M. Popelková, Z. Turanová, L. Koprdová, A. Ostró, S. Toporcerová, A. V. Makarevich, P. Chrenek
  • Journal:
    Zygote / Volume 17 / Issue 1 / February 2009
    Published online by Cambridge University Press:
    01 February 2009, pp. 57-61

  • The aim of the study was to determine the efficiency of two vitrification techniques followed by two assisted hatching (AH) techniques based on post-thaw developmental capacity of precompacted rabbit embryos and their ability to leave the zona pellucida (hatching) during in vitro culture. The total cell number and embryo diameter as additional markers of embryo quality after warming were evaluated. In vivo fertilized, in vitro cultured 8–12-cell rabbit embryos obtained from superovulated rabbit does were cryopreserved by two-step vitrification method using ethylene glycol (EG) as cryoprotectant or by one-step vitrification method with EG and Ficoll (EG+Ficoll). Thawed embryos were subjected to enzymatic or mechanical AH. Vitrified EG group showed significantly lower (P < 0.05) blastocyst rate (22.5%) and hatching rate (15%) than those vitrified with EG + Ficoll (63 and 63% resp.) and that of control (97 and 97% respectively). Significantly lower values of total cell number (P < 0.05) as well as embryo diameter (P < 0.01) in EG group compared with EG + Ficoll and control group were recorded. No significant difference was found in developmental potential of warmed embryos treated by either mechanical or enzymatic AH. The present study demonstrates that the EG + Ficoll vitrification protocol provides superior embryo survival rates over the EG vitrification protocol for 8–12-cell stage precompacted rabbit embryos. No positive effect of either mechanical or enzymatic AH on the post-thaw viability and quality of rabbit embryos in vitro was observed.