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It is necessary to make quantitative single-cell measurements of efficacy. Efficacy is broadly defined as anything that is helpful or is providing some kind of a positive therapeutic effect, either directly or indirectly, that can be done on a single-cell level. While traditional medical measures of efficacy remain relevant and useful, it is important for a nanomedical approach to remain focused mostly on what is happening at the single-cell level. These measurements may be structural or functional, but they allow us to determine the efficacy of nanomedical treatments at the single-cell level.
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