Search

Skip to main content Accessibility help

Only show content I have access to (0)

Chapters (1)

Last 3 years (1)

From year:

To year:

Apply

Medicine (1)

Cambridge University Press (1)

1 results

Chapter 4 - Extended Semen Analysis
David Mortimer, Lars Björndahl, Karolinska Institutet, Stockholm, Christopher L. R. Barratt, University of Dundee, José Antonio Castilla, Roelof Menkveld, Stellenbosch University, South Africa, Ulrik Kvist, Karolinska Institutet, Stockholm, Juan G. Alvarez, Trine B. Haugen
Book:

A Practical Guide to Basic Laboratory Andrology

Published online:

16 February 2022

Print publication:

10 March 2022, pp 81-113
- Chapter
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation
Summary

Provides methods for a range of common additional assessments on semen that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes anti-sperm antobodies (direct and indirect MAR test), immunocytochemical staining of leukocytes, meaaurement of reactive oxygen species and seminal plasma redox state, and biochemical tests on the seminal plasma to evaluate acessory gland function (zinc for the prostate, frutose for the seminal vesicles and alpha-glusidase for the epidiymis) and investigate abnormalities of the sequence of ejaculation. There is also a discussion on the microbiological examination of semen,.