The ability to monitor the welfare of animal collections in zoological institutions is critical to the mission of these facilities. Historically, zoos have utilised negative indicators of welfare, such as stereotypic behaviour to examine and monitor collection animals. However, absence of stereotypic behaviour or negative indicators of welfare does not indicate that an animal is thriving. The goal of the current study was to continue efforts to validate behavioural diversity as an indicator of welfare using cheetah (Acinonyx jubatus) as a model species. Behavioural and faecal glucocorticoid metabolite data were collected on 18 cheetah at the San Diego Zoo Safari Park over a period of three months to explore the relationship between behavioural diversity and adrenal hormones related to the stress response. Results suggest that behavioural diversity can be utilised as an indicator of animal welfare to monitor animal collections within zoological facilities. However, additional research with other species should be conducted to better understand behavioural diversity as a positive indicator of animal welfare. We hope this manuscript will increase discussion surrounding behavioural diversity as well as increase efforts to validate it as an indicator of welfare.