Aminocyclopyrachlor (AMCP) is labeled for use on zoysiagrass, but some injury has been observed. Differential zoysiagrass cultivar response to herbicide treatment has been previously reported. This greenhouse study evaluated the response of ‘BK-7’, ‘Cavalier’, ‘Emerald’, ‘Empire’, ‘Meyer’, and ‘Zorro’ zoysiagrass to 0, 0.005, 0.02, 0.11, 0.52, and 2.4, 11 kg ai ha−1, AMCP. Visual estimation of percent necrosis and normalized difference vegetative index (NDVI) analysis were conducted. Based on rating dates and data types three tolerance groups were established: Cavalier, Meyer, and Zorro are the most tolerant; Emerald and Empire are intermediate; and BK-7 is the least tolerant to AMCP. All zoysiagrass cultivars had sufficient tolerance at the labeled rate. Visual and NDVI analyses were highly correlated; however, NDVI data were subject to greater standard error and pseudo R2 values.