Tree rings were used to reconstruct mass balance for Peyto Glacier in the Canadian Rocky Mountains from A.D. 1673 to 1994. Summer balance was reconstructed from tree-ring estimates of summer temperature and precipitation in the Canadian Rockies. Winter balance was derived from tree-ring data from sites bordering the Gulf of Alaska and in western British Columbia. The models for winter and summer balance each explain over 40% of the variance in the appropriate mass-balance series. Over the period 1966–1994 the correlation between the reconstructed and measured net balances is 0.71. Strong positive mass balances are reconstructed for 1695–1720 and 1810–1825, when higher winter precipitation coincided with reduced ablation. Periods of reconstructed positive mass balance precede construction of terminal moraines throughout the Canadian Rockies ca. 1700–1725 and 1825–1850. Positive mass balances in the period 1845–1880 also correspond to intervals of glacier readvance. Mass balances were generally negative between 1760 and 1805. From 1673 to 1883 the mean annual net balance was +70 mm water equivalent per year (w.e./yr.), but it averaged −317 mm w.e./yr from 1884 to 1994. This reconstructed mass balance history provides a continuous record of glacier change that appears regionally representative and consistent with moraine and other proxy climate records.