The Habsburg Mausoleum in St. Vitus Cathedral in Prague, designed and constructed in the second half of the sixteenth century by Alexander Colin from Mecheln in the Low Countries, is often noted in modern scholarship as an early manifestation of the influence in Bohemia of the Habsburg dynasty, which had ascended the Bohemian throne in 1527 and ruled without interruption until 1918. Bridging both art historical and social cultural scholarship, this article explores the location and spatial features of the mausoleum, as well as its reception by contemporaries. It argues that while the style and size of the mausoleum is modest, its central location changed the dynastic symbolism in the cathedral, placing the Habsburgs at its center. And while it is less visible than other Habsburg cultural projects of its day, it—more than any single cultural project of the early Habsburg dynasty—demonstrates and symbolizes Habsburg ambitions in and commitment to Bohemia and is an important element in both the transformation of Prague into a Habsburg residential city and in long-term Catholic renewal efforts.