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semi-vivo

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1 results

Isolation of viable sperm cells from tobacco (Nicotiana tabacum)
Yajuan Cao, Adonis Reece, Scott D. Russell
Journal:

Zygote / Volume 4 / Issue 2 / May 1996

Published online by Cambridge University Press:

26 September 2008, pp. 81-84
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Viable sperm cells of Nicotiana tabacum were isolated by the semi-vivo technique. After pollination, excised styles were floated, cut end immersed, in a solution of 15% sucrose with 0.01% boric and 0.03% Ca(NO3)2 at 27°C in a growth chamber until pollen tubes emerged. After sperm cells were formed (at least 8 h after pollination) tubes were immersed in a 9% mannitol solution. In this solution, sperm cells are nearly ellipsoidal and retain viability for over 6 h.