We developed a new approach to identify vulnerabilities to water insecurity across entire archaeological culture areas by combining a paleohydrological model of the sensitivites of hydrological systems to droughts with least-cost analyses of the costs to acquire domestic water. Using a custom Python script integrated into ArcGIS Pro software, we calculated the pairwise one-way cost in time for walking between 225 water sources and 5,446 Ancestral Pueblo cultural sites across the Jemez and Pajarito Plateaus of the Jemez Mountains, New Mexico. This allowed us to identify whether periodic hydrological droughts occurring between AD 1100 and 1700 increased water acquisition costs across these regions. We found that hydrological droughts increased travel times in both regions to durations exceeding modern standards for water insecurity. Beginning in the fourteenth century, greater underlying hydrogeological sensitivities to droughts and the decline of a dual-residence pattern caused by population losses made the remaining aggregated communities of the Pajarito Plateau much more vulnerable to water insecurity than those on the Jemez Plateau. This would have upended long-standing relationships between communities and water on the Pajarito Plateau during a time when socioeconomic integration across the northern Rio Grande Valley pulled people toward valley bottoms.