Due to their different physiological effects, elevated carbon
dioxide and elevated ozone might have interactive
impacts on plants, and differentially so on plants differing in
photosynthetic pathway and growth rate. To test
several hypotheses related to these issues, we examined the
physiological, morphological and growth responses of
six perennial species grown at various atmospheric concentrations
of carbon dioxide and ozone. The species
involved (two C3 trees: Populus tremuloides Michx.,
Quercus rubra L.; two C3 grasses: Agropyron smithii
Rybd.,
Koeleria cristata L.; two C4 grasses: Bouteloua
curtipendula Michx., Schizachyrium scoparium Michx.) differed
in
growth form, stomatal conductance and photosynthetic pathway. In situ
photosynthesis, relative growth rate (RGR)
and its determinants (leaf area ratio, specific leaf area, leaf weight
ratio
and root weight ratio) were determined via
sequential harvests of seedlings that were grown in all combinations of
366 or 672 μmol mol−1
CO2 and 3 or 95 nmol mol−1 O3
over a 101-d period. Elevated CO2 had minimal effect on
either photosynthesis or RGR. By
contrast, RGR for all six species was lower in high
O3 concentrations at ambient CO2,
significantly so in A. smithii and P. tremuloides.
Five of the six species also exhibited reductions in in situ
photosynthesis at ambient CO2 in
high-O3-grown compared with low-O3-grown plants.
For
all species, these O3-induced reductions in RGR and
photosynthesis were absent in the elevated CO2 environment.
Root weight ratio was significantly reduced by
elevated O3 in A. smithii and P. tremuloides
in ambient but not elevated CO2. Species with high stomatal
conductance were the most susceptible to oxidant injury, while
those with low stomatal conductance, such as the
C4 species and Q. rubra, were not as detrimentally
affected by O3. Elevated levels of CO2 will reduce
stomatal
conductance and O3 uptake, and might therefore reduce the
potential for oxidant damage. However, there was a
stronger relationship of the percent reduction in whole-plant mass
due to O3, related to the ratio of photosynthesis
to stomatal conductance. In general, results of this study of six
functionally diverse plant species suggest that O3
pollution effects on carbon balance and growth are likely to be
ameliorated by elevated concentrations of atmospheric CO2.