The magnetobiostratigraphy study of the 130-m-thick Zújar section (Negratı́n clays unit, Guadix-Baza Basin, Spain) provides a remarkable opportunity to improve the correlation of European mammal biostratigraphy to the Geomagnetic Polarity Time Scale. The occurrence of 12 well-defined magnetozones and four paleontological sites with diagnostic faunas ranging from the MN 15 biozone (Ruscinian) to the MN 17 biozone (Villanyian), leads to an unambiguous correlation to chrons spanning from the Gilbert to the Matuyama epochs. This provides two new time constraints: (1) the boundary between MN 15 (Ruscinian) and MN 16 (Villanyian) biozones is recorded between chron C2An.3n and the base of chron C2An.2n and (2) the boundary between the upper and lower MN 16 subzones (Villanyian) is located between chron C2An.2n and the base of chron C2An.1n. The correlation between the Ruscinian–Villanyian boundary and the 3.3-myr-B.P. cooling event seems to be confirmed, while the early/late Villanyian boundary could be tentatively correlated to the glacial event at 2.6 myr B.P. Furthermore, MN 15 faunas are found in the lowermost part of chron C2Ar, which is in agreement with and reinforces the emplacement of the MN 14–MN 15 Ruscinian zones boundary at the reversal from chron 3n.1n to chron C2Ar.