Climbing beans play a central role in food security of rural households in the densely populated highlands of East and Central Africa. Soil fertility degradation and the lack of nutrient inputs are major limitations to yield of beans and other crops. We conducted field trials in Northern Rwanda in Kinoni and Muko villages to evaluate the effect of mineral N, P, and K fertilizers (both alone and in combination) and farmyard manure on nitrogen fixation and grain yields of climbing bean in smallholder farmers’ fields. The trials were laid down in a randomized complete block design with seven replicate blocks in each village. Manure and fertilizer application led to greater yields in all fields, and the largest yields were achieved when manure was combined with NPK. Large variability in yield between fields was observed. Application of fertilizer together with manure increased the grain yield from 1.5 to 3.9 t ha−1 in Kinoni and from 2.6 to 5.4 t ha−1 in Muko. Fertilizer and/or manure increased stover yield from 0.8 to 2.3 t ha−1 in Kinoni and from 1.5 to 3.4 t ha−1 in Muko. Application of 30 kg P ha−1 and 5 t manure ha−1 led to increased N and P uptake (from 49 to 106 kg N ha−1 and from 6.1 to 12.4 kg P ha−1 in Kinoni and from 46 to 128 kg N ha−1 and from 5.3 to 17.9 kg P ha−1 in Muko). There was no clear relationship between soil fertility characteristics and the response of climbing bean to applied inputs at Muko site. However, at Kinoni site, limited response to manure and NPK application was observed in plots where soil available P and soil exchangeable K were relatively low. Our results show the benefits of using manure along with mineral fertilizers for increased climbing bean yields and nutrient uptake in smallholder farming systems.

The mirid bug Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a major pest on cotton, fruit trees and other crops in China. A. lucorum adults often switch host plants in the agro-ecosystem, and such host-plant switching may promote more rapid population growth of A. lucorum. Here, we examined the population fitness of A. lucorum on different combinations of two plant foods [fresh maize kernels (Zea mays) and green bean pods (Phaseolus vulgaris)] in the laboratory when reared either individually or in groups. Our results suggested that, compared with A. lucorum nymphs reared on green bean alone, the survival rate, developmental rate, and adult weight significantly increased when they were fed fresh maize kernels for both rearing methods. Both two-plant combinations of foods (i.e., maize as nymphal food then green bean as adult food, and green bean as nymphal food then maize as adult food) generally prolonged adult longevity, improved female fecundity, and higher egg hatching rate compared with maize or green bean as food for both nymphs and adults. The combination of nymphs with maize and adults with green bean showed the highest population growth rate for both individual and group rearing of mirid bugs. Host food switching greatly promoted the population growth of A. lucorum, and suggests a new diet for laboratory rearing of A. lucorum.

Mexican bean weevil, Zabrotes subfasciatus Boheman, is a significant pest of stored beans (Phaseolus vulgaris L.) that causes considerable loss in the quality and quantity of bean grain in Africa. In the past two to three decades, researchers have attempted to identify botanicals with better insecticidal potential against storage pests. However, there is a lack of information on the potency of botanical combinations, their toxicology, and optimal application rates. This study was conducted to examine the bioefficacy of unitary and binary botanical combinations of Jatropha curcas (L.), Datura stramonium (L.), Chenopodium ambrosioides (L.), Schinus molle (L.), and Azadirachta indica (A. Juss) against Z. subfasciatus at 1% and 2% w/w dosages. The insecticidal activities of the botanicals were measured based on their effect on adult mortality of Z. subfasciatus and progeny reduction, grain weight loss, and weevil perforation index. Among botanical admixtures, binary combinations of C. ambrosioides + D. stramonium, J. curcas + C. ambrosioides, and S. molle + C. ambrosioides were the most potent. They resulted in the highest mortality of Z. subfasciatus, low weevil perforation index or none, and grain weight loss at the most moderate test dosage (1% w/w). Treatment with C. ambrosioides resulted in the highest efficacy when applied individually. We failed to observe any notable difference in toxicity between dosage levels among the binary botanical combinations. However, the effectiveness of unitary formulations improved with the increase in dosage rate. Application of botanicals did not adversely affect bean seed germination. Our study suggests that botanicals in binary mixtures may enhance the potency of constituent components for effective control of Z. subfasciatus.

Common bean (Phaseolus vulgaris L.) is amongst the most important grain legume crops in Africa in general, and Ethiopia in particular. The Mexican been weevil (Zabrotes subfasciatus Boheman) heavily attacks the grain of common bean. A total of 300 common bean entries were subjected to a ‘no-choice’ test at Melkassa Agricultural Research Centre, Ethiopia, using a randomized complete block design with three replications, to evaluate for resistance to the Mexican bean weevil. Data on insect and seed traits were collected and a significant level (P<0.01) of variation in all parameters measured was observed amongst genotypes. Relative resistance was recorded in landraces, improved genotypes and breeding lines, but the resistant genotypes, RAZ-11, RAZ-36, RAZ-2, RAZ-44, RAZ-120, RAZ-40 and MAZ-203, showed consistently complete resistance, with zero index of susceptibility value. Two other promising entries were also identified from the breeding lines (SCR-11) and landrace collections (NC-16) of Ethiopia. Stratified ranking diagrams showed that accessions from different eco-geographical origins in Ethiopia and those with different colours showed different patterns of response to infestation. The Ethiopian bean breeding programme should take up the resistant genotypes for a comprehensive yield trial at the national level and direct release them as commercial varieties. The incorporation of bean weevil resistance genes into adapted varieties through backcross breeding techniques, supported with marker assisted selection, seems to be the best strategy not only in terms of time saving but also in terms of effectiveness and efficiency.

Field experiments were conducted in 1987 and 1988 at Frederick, MD, to determine established horsenettle (Solanum carolinense L.) interference with snapbean. Horsenettle grown for 3 yr prior to planting snapbeans reduced yield 65% in 1987 and 48% in 1988. Horsenettle established for 1 yr reduced snapbean yield 20 and 18% in 1987 and 1988, respectively. As the distance of the snapbean row from the horsenettle row increased, there was less effect on the yield of snapbean. In 1987, snapbean yield in rows 15, 30, or 60 cm from horsenettle was reduced 43, 29, and 15%, respectively, while during 1988 in the same plots snapbean yields were reduced 38, 26, and 11%. Eight horsenettle planted in a 4.6-m row the first year and 16 horsenettle per row for the second year reduced snapbean yield 36 and 55% respectively. Horsenettle fruit number was 16% lower the first year and 45% lower the second year when snapbeans were grown at a 15-cm row spacing instead of a 60-cm row spacing.

The effects of photoperiod and temperature on the translocation of triclopyr {[1(3,5,6-trichloro-2-pyridinyl)oxy] acetic acid}, picloram (4-amino-3,5,6-trichloropicolinic acid) and 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid] were studied on tanoak [Lithocarpus densiflorus (Hook. & Arn.) Rehd.], snowbush ceanothus (Ceanothus velutinus Dougl.), bigleaf maple (Acer macrophyllum Pursh), bean (Phaseolus vulgaris L. var. ‘Red Kidney’) and barley (Hordeum vulgare L. var. CM-67). Isolation of 14C and analysis for the radioactive herbicides revealed little metabolism of the herbicides. Regardless of herbicide or plant species herbicide movement was greatest under warm temperature and long day conditions. Among the herbicides tested, 14C associated with triclopyr was the most mobile in each species. Each herbicide moved readily in the symplast but root applications of each herbicide revealed limited apoplastic mobility.

The organophosphate insecticides, malathion [S-1,2-di (ethoxycarbonyl)ethyl O, O-dimethyl phosphorodithioate], parathion (O, O-diethyl O-4-nitrophenyl phosphorothioate) and diazinon (O,O-diethyl O-2-isopropyl-6-methylpyrimidin-4-yl phosphorothioate) combined with bentazon [3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide], caused severe injury to soybean [Glycine max (L.) Merr. ‘Corsoy′] and navy bean (Phaseolus vulgaris L. 'Seafarer′). Postemergence tank – mixture applications of bentazon with organophosphate or carbamate insecticides, or soil-applied organophosphate insecticides prior to bentazon treatments, did not interact with bentazon to injure corn (Zea mays L. ‘Great Lakes Hybrid 4122′). Technical grade malathion interacted with bentazon to the same extent as formulated malathion. Combination treatments of malathion with bentazon resulted in the same degree of injury whether they were applied as tank mixtures or split applications 48 h apart.

Alfalfa (Medicago sativa L.), beans (Phaseolus vulgaris L.), corn (Zea mays L.), potatoes (Solanum tuberosum L.), spring wheat (Triticum aestivum L.), and sugarbeets (Beta vulgaris L.) were severely injured and had reduced foliage dry weights when grown in soil containing 0.10 ppmw or more terbacil (3-tert-butyl-5-chloro-6-methyluracil). Activated carbon at 150 kg/ha prevented a significant loss in weight of beans and corn in soil containing 0.10 ppmw terbacil and fully protected potatoes in soil containing 0.19 ppmw terbacil. Carbon at 300 kg/ha fully protected sugarbeets and prevented a significant weight loss in alfalfa in soil containing 0.19 ppmw terbacil. Carbon at 600 kg/ha did not protect wheat from 0.19 ppmw terbacil.

Of several herbicides tested on both monocotyledon and dicotyledon species, diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] was found to be the most effective in reducing transpiration in corn (Zea mays L.) plants; a reduction of 40% occurred. It was established that diuron was the active component in reducing transpiration, and not the additives in the commercial formulation (Karmex) used in this study. Foliar applications of low concentrations (0.035 to 0.56 kg/ha) of diuron effectively reduced transpiration in both cultivars (Pioneer 3780A and Pride 1108) of corn plants, at all stages of growth tested (from 3- to 10-leaf stage). This effect of diuron on reduced transpiration occurred when the supply of water or nutrients to the corn plants was either limiting or non-limiting. The reduction of transpiration of corn plants after treatment also prevented the leaves from wilting and becoming senescent. This effect was persistent over 16 days. Diuron treatment increased the water efficiency of the plants and this effect was more pronounced when water was limiting. Compared to the control, the treated plants used up to 68% less water for each gram of shoot dry weight produced. The increase in water efficiency indicates that transpiration was reduced more than photosynthesis.

A sprinkler system was developed that applied water to quadrant-shaped plots with a uniformity coefficient greater than 90% for any 2-m zone between 3 and 9 m from sprinklers that were located at the vertex of the quadrant. With this sprinkler system, many variables that affect the performance and behavior of herbicides applied in irrigation water can be studied. When applied through this sprinkler system in 1.4 cm of water, alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] at 3.3 kg/ha, EPTC (S-ethyl dipropylthiocarbamate) at 3.3 kg/ha, EPTC + trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) at 3.3 + 0.6 kg/ha, and EPTC + oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide) at 3.3 + 0.8 kg/ha controlled barnyardgrass [Echinochloa crus-galli (L.) Beauv.], common lambsquarters (Chenopodium album L.), and hairy nightshade (Solanum sarachoides Sendt.) selectively in beans (Phaseolus vulgaris L. ‘Pinto 114’).

In field experiments, chlorsulfuron and CGA 131036 did not injure winter wheat. Both herbicides applied postemergence at 9 and 10 g ai/ha completely controlled flixweed. CGA 131036 controlled cutleaf eveningprimrose 81%, and chlorsulfuron controlled 91%. At similar rates, CGA 131036 was also less effective than chlorsulfuron on henbit and Italian ryegrass. Both controlled wild buckwheat 77% when applied postemergence at 18 g/ha. Neither of the sulfonylurea herbicides controlled prostrate spurge or woolly croton as effectively as 2,4-D. In a bean root bioassay, root growth inhibition by both herbicides was partially alleviated by adding isoleucine and valine.

Applications of glyphosate [N-(phosphonomethyl)glycine] to the leaves of pea (Pisum sativum L. ‘Burpee's Blue Bantam’), sunflower (Helianthus annuus L. ‘Mammoth’), and bean (Phaseolus vulgaris L. ‘Red Kidney’) reduced transpiration in all three species. The reduction varied with species and concentration applied. Eight-day-old bean leaves responded earlier than leaves on older plants. However, after 29 h, there was no difference in transpiration rates with seedling age, since treated leaves on both ages investigated were transpiring at about one-half the rate of control leaves. Addition of surfactant to the solution shortened the time interval between treatment and transpirational response but did not affect the final transpiration rate as compared to glyphosate without surfactant. The effect of glyphosate on transpiration was not due to changes in water availability or to the surface of the leaf that was treated. It is suggested that these results can give some insight into uptake, translocation, and mode of action of glyphosate.

In four field experiments, acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid} applied preemergence at 1.1 kg/ha was not phytotoxic to peas (Pisum sativum L.) and increased the yield of shelled peas by an average of 25% compared to the weedy control. Yields were highest in all four experiments at the 1.1 kg/ha rate and tended to decline at higher rates of application. Growth chamber studies demonstrated that pea tolerance to acifluorfen was not affected by temperatures of 16/10 to 27/21C (light/dark) nor by stimulated rainfall of 0.5 to 5.0 cm. Peas and soybeans [Glycine max (L.) Merr.] were more tolerant of acifluorfen than were snap beans (Phaseolus vulgaris L.) and lima beans (Phaseolus lunatus L.) when acifluorfen was applied preemergence in the greenhouse or in hydroponic nutrient solution.

The effects of seed depth on growing point location of wild-proso millet, effects of alachlor placement, and chemical control of wild-proso millet were studied in field and greenhouse experiments. In 1986, 55 to 88% of the seedlings originating in the top 6 cm of soil had their growing point above the soil surface. The respective figures were 11 to 36% in 1987. Shoot exposure to alachlor resulted in significantly greater phytotoxicity than root exposure. Only EPTC or vernolate with a protectant, preplant incorporated, or pendimethalin preemergence, followed by atrazine plus tridiphane postemergence controlled wild-proso millet more than 85% all season.

This study describes separate methods for injecting growth retardants into herbaceous and woody plants. Bean plants (Phaseolus vulgaris L. ‘Black Valentine’) were treated by injecting growth retardant solutions into the hypocotyl cavity with a syringe. The chemical was subsequently absorbed into the vascular system. Woody California privet (Ligustrum ovalifolium Hassk.) was injected by forcing chemical solution into the vascular system via a predrilled hole. Results with NAA (1-napthalene-acetic acid) and MH (1,2-dihydro-3,6-pyridazinedione) validated the methods by inhibiting growth, respectively. New growth regulators, mefluidide {N-[2,4-dimethyl-5-[[(trifluoromethyl) sulfonyl]amino]phenyl]acetamide} and dikegulac (sodium salt of 2,3: 4,6-bis-O-(1-methylethylidene-α-L-xylo-2-hexulofuranosonic acid], inhibited the growth of bean with concentrations of 60 mg/L and 480 mg/L, respectively. Sprout growth of privet was inhibited by 30 mg/L of mefluidide and by 188 mg/L dikegulac.

Sublethal amounts of 2,4-D [(2,4-dichlorophenoxy) acetic acid] were applied to several vegetable crops in order to determine effect on yield and quality in a simulated drift experiment. Tomato (Lycopersicon esculentum Mill.) and root crops were most sensitive to 2,4-D; as little as 2.1 g/ha of acid distorted tomato fruit shape and elongated radish (Raphanus sativus L.) roots. All root crops were rendered unmarketable by 10.4 g/ha and gross yields were reduced by exposure to 104 g/ha. Yield of peppers (Capsicum frutescens L. var. grossum) was increased by exposure to 2.1 g/ha and severely depressed by 104 g/ha. Cucumber (Cucumis sativus L.) fruit shape was distorted at 11 g/ha and yield was reduced slightly. Lettuce (Lactuca sativa L.), onion (Allium cepa L.), and cabbage (Brassica oleracea L. var. capitata) were least sensitive to 2,4-D. Exposure to 20.8 g/ha did not reduce yields of lettuce or cabbage. Onion yields were reduced by application of 104 g/ha. Bush bean (Phaseolus vulgaris L.) yield was decreased by exposure to 22 g/ha, but potato (Solarium tuberosum L.) yield increased at 16 g/ha. Herbicide residues in the crop foliage were proportional to the degree of exposure. Residue analysis combined with foliar symptoms may be useful in predicting crop damage following 2,4-D drift.

In the field, eight barley (Hordeum vulgare L.) cultivars tolerated postemergence applications of AC 222,293 [a mixture of methyl 6 and 2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl) m- and p-toluate] at 0.4 to 1.4 kg/ha. AC 222,293 at 0.4 kg ai/ha applied at the 1.5- to 2-leaf stage controlled wild oats (Avena fatua L. # AVEFA) 96%, whereas 0.7 kg/ha applied at the 3.5- to 4-leaf stage controlled wild oats only 93%. Sugarbeets (Beta vulgaris L.) but not alfalfa (Medicago sativa L.), corn (Zea mays L.), pinto beans (Phaseolus vulgaris L.), or sunflower (Helianthus annus L.), were injured when planted 6 to 8 months after fall applications of AC 222,293 at 0.4 to 1.1 kg/ha.

Field trials were conducted in 1986 and 1987 to determine the effects of moisture on cinmethylin activity. The herbicide was applied preemergence at rates of 0.0, 0.3, 0.6, and 0.9 kg ai ha−1 to both dry and moist sandy loam soil, followed by varying irrigation regimes. Plant species used in the study included soybean, snap bean, cotton, peanut, and cucumber. Peanut was the most tolerant to cinmethylin, followed by cotton, soybean, snap bean, and cucumber. When cinmethylin was applied to a moist soil, less crop injury resulted than when it was applied to a dry soil. If 7.6 cm of water was applied shortly after cinmethylin application to a dry soil, severe crop injury occurred. When 2.5 cm of irrigation was applied within 8 h or at 5 days after cinmethylin application to a dry soil, crop injury was reduced when compared to applying 7.6 cm irrigation.

An economic analysis of four weed management systems employed on four crop sequences in a barley-corn-pinto bean-sugarbeet rotation in eastern Colorado was computed. Weeds were controlled in each crop with only conventional tillage or conventional tillage plus minimum levels of herbicides (systems 3 and 4), moderate levels of herbicides (system 1), or intensive levels of herbicides (system 2). Adjusted gross returns were higher for systems 3 and 4 where herbicide use was less/year and decreased over 4 yr than for systems 1 and 2 where herbicide use was higher/year and constant. When the four crop sequences were aggregated using yield and sucrose indices, the least herbicide-intensive weed management system had $440/ha/4 yr higher indexed adjusted gross return than the most herbicide-intensive weed management system. An income risk analysis showed that the herbicide-intensive weed management system was not risk efficient and that producers would select one of the other three less herbicide-intensive weed management systems depending upon their risk preferences.

The applicability of fluorescence measurements for the detection of herbicide effects in whole leaves was analyzed. Based on the results known for isolated chloroplasts, normalized variable fluorescence of the initial rise was shown to be an appropriate tool for monitoring effects of photosystem II (PS II) herbicides. Equipment is described for monitoring the degree of inhibition by fluorescence induction measurements and microcomputer data analysis. The method is used to study the effect of pyrazon [5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone], BAY DRW 1139 [4-amino-3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one], and phenmedipham {3-[(methoxycarbonyl)amino] phenyl (3-methyl-phenyl)carbamate} after foliar application to different species. A rapid decrease of normalized variable fluorescence indicates penetration into leaf cells of all species tested. During a 5- to 7-day experiment, the apparent variable fluorescence decreased continuously in herbicide-susceptible plants, while it recovered in resistant plants due to an internal detoxification mechanism. The described method provides a rapid, simple, and nondestructive tool for analyzing the kinetics of penetration and detoxification of PS II herbicides in whole leaves.