Botanical microfossils, macroremains and oribatid mites of a Weichselian interstadial deposit in the central Netherlands point to a temporary, sub-arctic wetland in a treeless landscape. Radiocarbon dates and OSL dates show an age between ca. 54.6 and 46.6 ka cal BP. The vegetation succession, starting as a peat-forming wetland that developed into a lake, might well be linked with a Dansgaard-Oeschger climatic cycle. We suggest that during the rapid warming at the start of a D-O cycle, relatively low areas in the landscape became wetlands where peat was formed. During the more gradual temperature decline that followed, evaporation diminished; the wetlands became inundated and lake sediments were formed. During subsequent sub-arctic conditions the interstadial deposits were covered with wind-blown sand. Apart from changes in effective precipitation also the climate-related presence and absence of permafrost conditions may have played a role in the formation of the observed sedimentological sequence from sand to peat, through lacustrine sediment, with coversand on top. The Wageningen sequence may correspond with D-O event 12, 13 or 14. Some hitherto not recorded microfossils were described and illustrated.