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oocyte cytoskeleton

Zygote (1)

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Intermediate filament proteins immunologically related to cytokeratins in the oocyte of the fish Cyprinus carpio
Caterina Mencarelli, Franco Cotelli
Journal:

Zygote / Volume 5 / Issue 3 / August 1997

Published online by Cambridge University Press:

26 September 2008, pp. 207-212
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We have used monoclonal antibodies specific for different sets of human cytokeratins and the anti-IFA (Intermediate Filament Antigen) antibody to investigate the expression of intermediate filament proteins in the mature oocyte of the teleostCyprinus carpio. Several polypeptides have been identified, showing molecular weights ranging from 43 to 65kDa. Two-dimensional analysis of the immunoreactive species revealed the presence of at least six major protein spots and a series of minor components, grouped in quite a narrow pI range from 5.52 to 6.28. The general complexity of the carp oocyte cytokeratin-related cytoskeleton appears to be higher than those described for oocytes of other vertebrate species.