Oestrogen–progesterone signalling is highly versatile and critical for the maintenance of healthy endometrium in humans. The genomic and nongenomic signalling cascades initiated by these hormones in differentiated cells of endometrium have been the primary focus of research since 1920s. However, last decade of research has shown a significant role of stem cells in the maintenance of a healthy endometrium and the modulatory effects of hormones on these cells. Endometriosis, the growth of endometrium outside the uterus, is very common in infertile patients and the elusiveness in understanding of disease pathology causes hindrance in selection of treatment approaches to enhance fertility. In endometriosis, the stem cells are dysfunctional as it can confer progesterone resistance to their progenies resulting in disharmony of hormonal orchestration of endometrial homeostasis. The bidirectional communication between stem cell signalling pathways and oestrogen–progesterone signalling is found to be disrupted in endometriosis though it is not clear which precedes the other. In this paper, we review the intricate connection between hormones, stem cells and the cross-talks in their signalling cascades in normal endometrium and discuss how this is deregulated in endometriosis. Re-examination of the oestrogen–progesterone dependency of endometrium with a focus on stem cells is imperative to delineate infertility associated with endometriosis and thereby aid in designing better treatment modalities.

In this paper, numerical study was systematically conducted to analyze the shear banding evolution in bulk metallic glasses (BMGs) with various notches subjected to the uniaxial compression, and the relation between the notched configurations and compressive malleability was therefore elucidated. Free volume was used to be an internal state variable to depict the shear banding nucleation, growth and coalescence in the BMGs with the aid of free volume theory, which was incorporated into ABAQUS finite element method code as a user material subroutine. The present numerical procedure was firstly verified by comparing with the existing experimental data, and then parameter analysis was performed to discuss the impacts of notch shape, notch size, notch orientation, and notch configuration on the plastic deformability of notched samples. The present modeling will shed some light on the failure mechanisms and the toughening design of notched BMG structures in the engineering applications.

In the present study, an ultra-wideband (UWB) antenna has been proposed using coplanar waveguide (CPW) feed with dual-band-notch characteristics. Slot-loaded radiator and U-shaped CPW resonator are used for band rejection at 3.5 and 5–6 GHz respectively to reduce interference with existing World interoperability for microwave access and wireless local area network systems. With an extended operating band (measured at 10 dB return loss) the antenna operates successfully over the entire UWB range (3.1–10.6 GHz) with a form factor of 30 × 20 × 1.524 mm3 on a commercially low-cost FR-4 substrate. Experimental measurement results are presented in support of the simulated results for the proposed antenna for practical application. The antenna has been successfully fabricated and measured, showing broadband matched impedance and good omnidirectional radiation pattern throughout the operating bandwidth. Measured time-domain analysis for both the orientations, i.e. face-to-face and side-by-side, yields excellent performance in the open environment scenario. With fairly good and consistent monopole such as omnidirectional radiation patterns in H-plane and linear transmission responses, the proposed antenna is well suited to be integrated within portable devices.

The temperature versus life (T-N) curves of both centrally notched and unnotched AS-4/PEEK (APC-2) composite laminates due to constant stress amplitude tension-tension (T-T) fatigue loading were investigated systematically. The cross-ply laminate possesses the higher mechanical properties than those of the quasi-isotropic laminate at elevated temperature, and also the mechanical properties of both lay-ups are degraded significantly as the temperature increasing. Combining both effects of notch and temperature at various normalized stresses it is found the cross-ply laminate possesses more resistance to cyclic loading than that of the quasi-isotropic laminate. Additionally, the predictive strain model is in a practical and simple form. Alternatively, the T-N curves, instead of conventionally S-N curves, at variously applied stresses are accomplished for preliminary designs and applications.

The FEM is used to study the effects of notch opening angle (β) and notch radius on the new strain-concentration factor (SNCF) for circumferentially V-notched cylindrical bars under static tension. The new SNCF has been defined under the triaxial stress state at the net section. Nevertheless, the conventional SNCF has been defined under uniaxial stress state. The new SNCF () is constant in the elastic deformation. The range where this elastic value remains constant increases with increasing β and increasing notch radius (ρo). The effect of the notch opening angle on the elastic decreases with increasing ρo. Particularly, the elastic of β = 120° is the minimum for all notch radii employed. The new SNCF increases from its elastic value to a peak value and then decreases with plastic deformation for notches with β = 120°. This peak value is the maximum . Nevertheless, for the notches with β < 120° becomes nearly constant or slightly decreasing after the first peak for ρo = 0.5 and 1mm. After that it increases to the maximum SNCF and then slightly decreases for further plastic deformation. The variations in with the ratio of tensile load to that at yielding at the notch root (P/PY) are nearly independent of stress-strain curve up to general yielding.

Recently, adult stem cells have become a focus of intensive biomedical research, but thecomplex regulation that allows a small population of stem cells to replenish depleted tissues is stillunknown. It has been suggested that specific tissue structures delimit the spaces where stem cellsundergo unlimited proliferation (stem cell niche). In contrast, mathematical analysis suggests thata feedback control of stem cells on their own proliferation and differentiation (denoted QuorumSensing) suffices for preserving homeostasis in developing tissues. In the present work we examinethis discrepancy by analyzing a mathematical model of signalling pathways in a mammary stemcell. Our work shows that the model has a unique equilibrium depending on the environmentalconditions, which is stable for appropriate parameter values. We show how this dependence onthe micro-environment can control the cell decision to either proliferate or differentiate. Our workfurther suggests that this description is sufficient for the creation of niche conditions, with no needof additional structures. The analysis also pinpoints the pathways that may serve as the QuorumSensing mechanism.

Fish can regenerate retinal neurons following ocular injury. Evidence is mounting that astrocytic glia function as inducible, regenerative stem cells in this process, but the underlying molecular events that enable neuronal regeneration are comparatively unclear. In the current study gene array, quantitative real-time PCR, in situ hybridization, and immunohistochemical approaches were used to identify, in the damaged retina of adult zebrafish, correlations between transcriptional events and entry into the cell cycle by Müller cells, a type of astrocytic cell present in all vertebrate retinas that is a candidate ‘stem cell’ of regenerated neurons. A proneural gene (achaete-scute homolog 1a, ash1a) and neurogenic components of the Notch signaling pathway, including notch3 and deltaA, were implicated. An injury-induced, enhanced expression of ash1a was observed in Müller cells, which is hypothesized to contribute to the transition of these cells, or their cellular progeny, into a notch3-expressing, regenerative progenitor. A model of vertebrate retinal repair is suggested in which damage-induced expression of proneural genes, plus canonical Notch-Delta signaling, could contribute to retinal stem cell promotion and subsequent regenerative neurogenesis.

Both neurons and glia of the PNS are derived from the neural crest. In this study, we have examined the potential function of lunatic fringe in neural tube and trunk neural crest development by gain-of-function analysis during early stages of nervous system formation. Normally lunatic fringe is expressed in three broad bands within the neural tube, and is most prominent in the dorsal neural tube containing neural crest precursors. Using retrovirally-mediated gene transfer, we find that excess lunatic fringe in the neural tube increases the numbers of neural crest cells in the migratory stream via an apparent increase in cell proliferation. In addition, lunatic fringe augments the numbers of neurons and upregulates Delta-1 expression. The results indicate that, by modulating Notch/Delta signaling, lunatic fringe not only increases cell division of neural crest precursors, but also increases the numbers of neurons in the trunk neural crest.

Prospero is required in dividing longitudinal glia (LG) during axon guidance; initially to enable glial division in response to neuronal contact, and subsequently to maintain glial precursors in a quiescent state with mitotic potential. Only Prospero-positive LG respond to neuronal ablation by over-proliferating, mimicking a glial-repair response. Prospero is distributed unequally through the progeny cells of the longitudinal glioblast lineage. Just before axon contact the concentration of Prospero is higher in two of the four progeny cells, and after axon guidance Prospero is present only in six out of ten progeny LG. Here we ask how Prospero is distributed unequally in these two distinct phases. We show that before neuronal contact, longitudinal glioblasts undergo invaginating divisions, perpendicular to the ectodermal layer. Miranda is required to segregate Prospero asymmetrically up to the four glial-progeny stage. After neuronal contact, Prospero is present in only the LG that activate Notch signalling in response to Serrate provided by commissural axons, and Numb is restricted to the glia that do not contain Prospero. As a result of this dual regulation of Prospero deployment, glia are coupled to the formation and maintenance of axonal trajectories.

a significant proportion of the human population suffers from some form of skin disorder, whether it be from burn injury or inherited skin anomalies. the ideal treatment for skin disorders would be to regrow skin tissue from stem cells residing in the individual patient's skin. locating these adult stem cells and elucidating the molecules involved in orchestrating the production of new skin cells are important steps in devising more-efficient methods of skin production and wound healing via the ex vivo expansion of patient keratinocytes in culture. this review focuses on the structure of the skin, the identification of skin stem cells, and the role of notch, wnt and hedgehog signalling cascades in regulating the fate of epidermal stem cells.