Five Graphis species are reported as new to Europe from the Mata Nacional do Buçaco, a region in Portugal where graphidoid Graphidaceae are abundant and diverse. The following species were identified: Graphis dendrogramma, G. duplicata, G. gonimica, G. librata and G. pyrrhocheiloides. An updated key is given to the Graphis species known from Europe. All those newly reported were identified with names corresponding to tropical species.