Operational deep-sea manned submersibles are important underwater vehicles that provide strong technical support for international deep-sea research. Navigation and positioning technologies are expected to facilitate the complete exploitation of the scientific value of samples and data collected by such submersibles, improve their underwater operation efficiency and enhance their safety. This paper first briefly describes six operational deep-sea manned submersibles that can dive to depths of more than 4,500 m. Then, the paper focuses on the navigation and positioning technologies used in these submersibles. Finally, considering the existing problems in the navigation and positioning systems of China's manned submersible Jiaolong, the paper discusses future development trends of the navigation and positioning technologies used in operational deep-sea manned submersibles.