Inside Manitou Cave in modern Alabama, nineteenth-century Cherokees carried out sacred ceremonies, recording their activities on the walls using Cherokee syllabary, a system invented in nearby Willstown by Cherokee scholar Sequoyah. Through collaboration between modern Cherokee scholars and Euro-American archaeologists, the authors report and interpret—for the first time—the inscriptions in Manitou Cave. These reveal evidence for secluded ceremonial activities at a time of crisis for the Cherokee. Pressures from the surrounding white populations disrupted the Cherokee ancient lifeways, culminating in their forcible relocation in the 1830s along the Trail of Tears.