Preserving wildlife and their environment from anthropogenic activities requires identification and establishment of protected areas, and monitoring of their long-term effects on wildlife and habitat. Tropical forests are one of the most at-risk habitats and many tropical species have become extinct recently due to human activity. It is imperative to monitor habitat in protected areas and without in order to identify strategies and legislative policies that optimize conservation outcomes. To this end, I quantified habitat fragmentation for the great hornbill (Buceros bicornis) in Om Koi Wildlife Sanctuary, Thailand, pre- and post-establishment, within and outside the protected area, from 1973, 1985, and 1992, to assess the effectiveness of the protected area status, established in 1978, and a national logging ban, established in 1989, in preserving and restoring hornbill habitat. The results demonstrate that the establishment of Om Koi Wildlife Sanctuary did not decrease the rate of hornbill habitat fragmentation relative to areas outside the protected area. While the protected area had less fragmentation to begin with, protection status did not affect the rate of loss. Fragmentation increased significantly both inside and outside the protected area between the first and second time points (p < 0.05), after the protected area was first established. However, the national logging ban policy implemented in 1985 seems to have successfully halted the fragmentation of habitat within the protected area and surrounding unprotected areas, with all areas showing no significant change (p > 0.05). While not significant, the rate of fragmentation outside the protected area was greater. This suggests that the establishment of a protected area alone may not be sufficient to stop or reverse anthropogenic damage to endangered habitat and the species that utilize these environments. The incorporation of multiple strategies for management is likely needed to increase the ability of protected areas to preserve tropical forest species and habitats. The assessment of protected areas via satellite and ground-level data is an essential tool for evaluating the effectiveness of conservation strategies and improving outcomes.