Food is an important factor for the survival of juvenile fish. Knowledge of the diet of co-existing species helps clarify their relationships with each other. A number of Mugilidae species are known to co-exist in estuarine systems, raising the question of whether they compete for food resources. The feeding behaviours of five juvenile Mugilidae species were studied in the estuaries of Strymonikos (North Aegean Sea) using stomach content analysis. It was found that the species Chelon labrosus, Liza saliens and Mugil cephalus presented high feeding activity during summer and autumn and L. ramada and L. aurata during winter and spring. The diet overlap between the species was generally moderate to low and the pair L. saliens – M. cephalus exhibited the highest overlap in Richios estuarine system. The species do not appear to compete for common resources, probably because there is not always a spatiotemporal overlap, enough food is available or they exploit different resources. Four out of the five species exhibited similar patterns of feeding strategies with varying levels of specialization at an individual level and a rather generalized pattern at the population level. This more generalized feeding strategy may permit them to co-exist. Only M. cephalus showed a more specialized feeding behaviour, with a strong preference for microalgae. The absence of a general pattern for the trophic levels according to seasons, sizes or locations, also imply the opportunistic character of the species. Both season and fish size influenced the variation in the diet composition.