Since 2003, as part of the LIFE project ‘Del.Ta.’ (NAT/IT/000163) a bottlenose dolphin community has been studied in order to assess the impact of increasing boat traffic in the Pelagie Archipelago (Sicily, Italy). During the summer of 2006 and 2008, land-based surveys were carried out at Lampedusa, one of the Archipelago's Islands, at set hours from 6 locations (7 in 2008) in standard weather conditions. Continuous horizon scanning and focal group sampling methods were used to study dolphin presence and behaviour. Dolphin–boat interactions were recorded for each sighting. A total of approximately 559 hours were spent monitoring and 83 sightings were recorded. With regard to the different types of vessels, interaction with sailing boats was always neutral, whereas fast boats caused the interruption of all activities and avoidance behaviour. Positive interaction occasionally occurred with bottom trawling fishing boats. Interactions recorded when fishing and powered engine boats approached within 200 m predominantly resulted in a negative reaction from the dolphins (approximately 70%) and dolphin behaviour was affected in terms of occurrence and patterns. Behavioural analysis showed that the animals reduced the frequency of behaviours related to feeding, social and relaxing activities. The dolphins also increased information-gathering behaviour such as spyhopping. The use of Markovian chain models revealed evidence of disruptions in sub-sequences made up of behaviours often associated with feeding. After boat departures, the animals rarely restarted their previous activity and persistence of this effect could have long-term consequences as a result of non-habituation. Sighting duration also decreased in the presence of vessels. The possible negative long-term effect of the presence of boats may be indicated by the lower mean sighting duration in 2008, when the highest presence of powered engine boats was recorded. In view of the scenario outlined above, local authorities need to enforce the requirement of maintaining a distance of over 200 m away from the animals for all types of boat and also promote regular land-based surveys of vessel impacts for tourist boats and dolphin watching cruises.