The mixing behavior of a two-channel micromixer with a circular mixing chamber at four different chamber depths and six different flow rates had been investigated. Experiments were implemented with the mixings of two fluids. An image inspection method using the variance of the image gray level contrast as the measurement parameter to determine the mixing efficiency distribution in these mixers. The steady, three-dimensional and laminar flow fields inside the micromixers were also simulated numerically with a finite volume discretization. Through the numerical integration over the chamber depth, the three-dimensional numerical prediction could be compressed into a two-dimensional result, which could be directly used to compare with the experimental measurements. Experimental results show that the measured mixing efficiency is raised with the increase of chamber depth. The numerical prediction of mixing efficiency agreed qualitatively with those obtained from the experimental measurements, while the ratio of the depth to diameter of the mixing chamber is big enough to eliminate the viscosity effect.