The evolution processes and forcing mechanisms of the Horqin dunefield in northern China are poorly understood. In this study, systematic OSL dating of multiple sites is used together with pollen analysis of a representative section in order to reconstruct the evolution of the dunefield since the Last Glacial Maximum (LGM). Our results show that there was extensive dune mobilization 25–10 ka, transition to stabilization 10–8 ka, considerable dune stabilization 8–3 ka, and multiple episodes of stabilization and mobilization after 3 ka. Comparison of dune evolution of the dunefields in northern China during the Holocene showed that Asian monsoon and resultant effective moisture have played an important role in the evolution of dunefields at the millennial time scale. Further analysis indicated that the dune evolution in the Horqin dunefield before 3 ka was synchronous with climatic changes. However, increasing human activity has impacted dune evolution during the last 3 ka.