Risk of developing the metabolic syndrome may be influenced by nutritional environment early in life. We examined the effects of high-fibre (HF) and high-protein (HP) diets consumed during pregnancy and lactation on satiety hormones and expression of genes involved in glucose and lipid metabolism in offspring. Wistar dams were fed a control (C), HF or HP diets during pregnancy and lactation. At parturition, litters were culled to ten pups. At 21 d, all pups were weaned onto C diet. At 7, 14, 21, 28 and 35 d after birth, blood was analysed for satiety hormones and tissues for mRNA expression in offspring. No differences were observed in litter size or birth weight. At 21 d, offspring of HF dams had greater adjusted intestinal mass and lower liver weight than those of C but not of HP dams. Plasma glucose at 28 d and amylin at 7, 14 and 28 d were lower in HF v. C and HP offspring. Glucagon-like peptide-1 was higher in HP offspring than in HF offspring at 7 d but was higher in HF v. C offspring at 21 d. Offspring of HF dams had higher glucose transporter (GLUT2 and Na+-dependent glucose/galactose transporter) mRNA expression at 21 d v. C and HP offspring. In brown adipose tissue, HF and HP up-regulated uncoupling protein-1 and PPAR-γ coactivator. HP was associated with increased resistin and IL-6 mRNA expression. The present study demonstrates that maternal diet composition differentially regulates circulating satiety hormones and genes involved in glucose transport and energy metabolism in offspring. These early changes could have long-term consequences for obesity risk.

A low (L) and high-fibre (H) diet were fed to six multiparous sows during gestation in a 2 × 2 repeated Latin square design. A single meal per day was given that provided 37·2 MJ digestible energy/d. The kinetics of heat production (HP) and its partitioning (fasting HP, activity HP, and thermic effect of feeding (TEF)) were determined. The TEF was partitioned between a dynamic component (TEFst) and a constant component (TEFlt). Digestibility of energy and nutrients was lower for the diet H. In spite of the lower metabolisable energy (ME) intake (33·9 v. 35·4 MJ/d for diets H and L respectively), HP was higher for diet H (30·5 v. 28·9 MJ/d) resulting in a lower energy retention. The estimated fasting HP was 270 kJ/kg body weight0·75 per d at day 0 of gestation and increased with advancement of pregnancy. The TEFlt was not significantly different from zero for diet L, but represented 4·1 % of ME intake for diet H. The TEFst was not affected by the diet but diet H delayed the postprandial peak of HP. Total TEF was higher for diet H than for diet L (11·7 v. 8·2 % of ME intake). The longer duration of eating with diet H was compensated for by less physical activity between meals, so that activity HP was equivalent for both diets. The activity HP represented 20 % of ME intake but was variable between sows. The ME requirements for maintenance averaged 440 kJ/kg body weight0·75per d. Feeding high-fibre diets increases HP, delays the postprandial peak of HP and maintains the basal HP at a higher level.