Cover crop systems were investigated in 2004 and 2005 for their effects on the activity-density (a function of movement and density) of a promising group of weed biocontrol organisms, the ground beetles collectively known as carabids, with particular emphasis on a beneficial carabid species Harpalus rufipes DeGeer. Marked H. rufipes released into pea/oat–rye/vetch cover crop plots were more than twice as likely to be recaptured within the same plots as beetles released in nonvegetated fallow plots (18 and 8%, respectively). Marked beetles released into fallow plots were more than twice as likely to leave their plots and be recaptured in pea/oat–rye/vetch plots as vice versa (13 vs. 5%), indicating a clear preference for habitat with vegetative cover. Overall recapture rates were not different between treatments. Unmarked H. rufipes activity-density was also higher in pea/oat–rye/vetch compared to fallow plots. Additionally, five cover crop systems, including the fallow and pea/oat–rye/vetch treatments, and two residue management methods (conventional and zone tillage) were investigated from June to August in 2005 for their effects on H. rufipes activity-density. Corn was planted in 2005 into residues of the five cover crop systems grown in 2004. H. rufipes activity-density was higher in zone and conventionally tilled corn planted in pea/oat–rye/vetch residues and conventionally tilled corn planted in red clover/oat residues than in any other cover crop and residue management combination. Pea/oat–rye/vetch cover crop systems are apparently beneficial for H. rufipes during the cover crop year as well as in subsequent crops planted into this cover crop's residues. This system was not the least disturbed system but, based on the number of tillage events, represented a medium level of disturbance among the various systems. Thus, some level of disturbance might be beneficial for H. rufipes, but how and when that soil disturbance occurs requires further research to determine the best means of conserving this species.

Contrasting cover-cropping systems were compared to determine whether fundamental differences in cover-cropping strategies affect weed seed predators and resulting seed predation. We conducted typical “feeding” trials in which 25 seeds of each of six weed species, including velvetleaf, wild mustard, yellow foxtail, common lambsquarters, redroot pigweed, and hairy galinsoga, were placed in the field. Exclosures showed that the majority of seed predation could be attributed to invertebrates: 43% out of a total of 56% seed predation for 11 d in 2002 and 40% out of a total of 58% seed predation for 4 d in 2003. The predominant invertebrate seed predator across all entry points of four cropping systems was a ground-dwelling carabid beetle, Harpalus rufipes, which was more abundant in vegetated treatments, particularly red clover, compared with treatments recently tilled and planted to a fall cover crop. In the absence of vertebrates, H. rufipes activity–density was positively correlated with mean seed predation in 2002 (Spearman ρ = 0.489; P < 0.001) but not in 2003 (Spearman ρ = 0.090; P = 0.504), possibly because of a delay between pitfall trapping and predation assay. The activity–density of invertebrate seed predators measured in these systems and the high level of predation imposed on weed seeds at the soil surface indicate that cover-cropping strategies should consider late-season weed management, which maintains seeds on the soil surface and provides desirable habitat for invertebrate predators.

Granivory can play a pivotal role in influencing regeneration, colonization as well as abundance and distribution of plants. Due to their high abundance, nutrient content and longevity, seeds are an important food source for many animals. Among insects, carabid beetles consume substantial numbers of seeds and are thought to be responsible for a significant amount of seed loss. However, the processes that govern which seeds are eaten and are therefore prevented from entering the seedbank are poorly understood. Here, we assess if DNA-based diet analysis allows tracking the consumption of seeds by carabids. Adult individuals of Harpalus rufipes were fed with seeds of Taraxacum officinale and Lolium perenne allowing them to digest for up to 3 days. Regurgitates were tested for the DNA of ingested seeds at eight different time points post-feeding using general and species-specific plant primers. The detection of seed DNA decreased with digestion time for both seed species, albeit in a species-specific manner. Significant differences in overall DNA detection rates were found with the general plant primers but not with the species-specific primers. This can have implications for the interpretation of trophic data derived from next-generation sequencing, which is based on the application of general primers. Our findings demonstrate that seed predation by carabids can be tracked, molecularly, on a species-specific level, providing a new way to unravel the mechanisms underlying in-field diet choice in granivores.