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germ-line chimaerism

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1 results

Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish
M. Francisco-Simão, J. Cardona-Costa, M. Pérez-Camps, F. García-Ximénez
Journal:

Zygote / Volume 19 / Issue 1 / February 2011

Published online by Cambridge University Press:

23 June 2010, pp. 67-70
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The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.