The objective of this study is to prospect ISSR primers (Inter Simple Sequence Repeats) and, further genetic characterization in Paratecoma peroba. For this, leaf samples of 20 individuals were collected in a forest fragment, located in a private area, close to the Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo, in the city of Alegre, ES, Brazil. For this, 43 ISSR primers were tested, and 10 primers were selected, which provided a total of 91 bands, with 57 polymorphic bands, resulting in 62.63% polymorphism. The polymorphic information content (PIC = 0.27) indicated moderate informativeness of the primers and, therefore, they are efficient in studies with the species. However, the values found for genetic parameters such as the number of observed (AO = 1.68) and effective (AE = 1.41) alleles and, the genetic diversity indices of Nei (H* = 0.23) and Shannon (I* = 0.35) indicate the occurrence of homozygous loci and low genetic diversity in the population. On the other hand, the genetic structure evaluated by the Bayesian approach revealed the formation of three genetic groups distributed in all sampled individuals, inferring once again about the occurrence of loci in homozygosity. Therefore, the connection of neighbouring fragments and the establishment of individuals obtained from other sources could increase the genetic diversity of the population and reduce the possible effects of depression by inbreeding and genetic drift.

Crop wild relatives (CWR) are valuable resources for crop breeding due to their close genetic relationship to the cultivated plants and their wide genetic variation, allowing the introgression of desirable traits into the crops, such as resistance to plant pests and diseases or adaptability to climate change. Mexico is a centre of agrobiodiversity, including CWR, but climate change, and other factors, are contributing to the loss of important Mexican CWR genetic diversity. The in situ and ex situ conservation status of Mexican priority CWR were assessed through a gap analysis as part of a national CWR conservation strategy for Mexico, to ensure the long-term preservation and improve the availability of these genetic resources. A set of 310 priority CWR taxa, previously identified as part of the national CWR inventory for Mexico, were analysed. Species distribution modelling and ecogeographic diversity analyses were used to detect gaps in in situ and ex situ conservation at taxon and ecogeographic levels. Priority target sites were identified throughout the country for complementary in situ and ex situ conservation of these taxa. The results obtained allow us to make recommendations for immediate conservation actions, thus helping to mitigate the threats to Mexican agrobiodiversity and enhance both national and global food security.

Rhazya stricta is an endangered medicinal plant that is under severe human pressure as a result of commercial harvesting. As a model of habitat fragmentation, we selected the Kohat Plateau in the Pakistani Himalaya, where populations are fragmented, with less than 100 individuals per population. We hypothesized that the populations may be threatened by rapid habitat fragmentation and by unsustainable utilization of the plant. We analysed P450-based analogue functional genomic markers and amplified fragment length polymorphism markers from six populations of R. stricta on the Kohat Plateau, and examined their variations both within and among the populations. Both the marker groups revealed lower genetic differentiation among the populations and higher genetic differentiation within the populations as a result of high gene flow. The results confirmed that habitat fragmentation is being caused by severe human pressure, and although signs of genetic erosion are not yet visible, they seem likely to become visible in the future. Therefore, the conservation of R. stricta populations is necessary.

Six lines of laying chickens representing high-producing non-industrial lines chosen or produced with consideration for characters of production are being kept at the Agassiz Research Centre. The collection includes one Barred Plymouth Rock (Line 60), one Columbian Plymouth Rock, one Rhode Island Red (Line 50), and three White Leghorn lines (Blue, Black, and Burgundy). Before coming to the Agassiz Research Centre these pure lines were subjected to mild selection for egg production and their egg production approaches that of commercial hybrids. The lines are currently maintained as a genetic resource as live populations without selection and with populations large enough to minimize inbreeding. In addition, samples of DNA from these lines have been conserved for genetic studies, and samples of embryonic cells are being kept cryogenically to allow the possibility of reconstitution of the lines through the production of germline chimeras. Techniques of ovarian transplantation are being developed which will allow more efficient cryogenic conservation and recuperation of the genetic material into live populations.

Le rapport Danchin/Lauvergne de mai 2000 qui traitait de la situation actuelle de la chèvre du Rove est analysé par un acteur passionné de sauvegarde de la race. La Rove est une chèvre qui, en Provence, accompagnait jadis les troupeaux ovins transhumant à pied. Pour sa sauvegarde deux axes de travail sont actuellement privilégiés: 1) l'accès aux primes pour les bergers des troupeaux qui nettoient avec leurs dents les pare-feux forestiers permettant l'accès des intervenants contre les feux de forêt et 2) la recherche de débouchés pour la viande de cabris lourds, unique produit commercialisable puisque les chèvres débroussailleuses ne produisent pas de lait. Des solutions sont en vue avec la mise en place de CTE (contrats territoriaux d'exploitation) pour le premier point et la montée en puissance d'une entreprise bouchère locale pour le second. Toutes ces actions vont dans le sens d'une gestion écologique et économique bien comprise des troupeaux Rove existant encore. Elle combine la conservation d'une ressource génétique domestique, une contribution à la protection de la forêt contre le feu, la production d'une viande saine car élaborée à partir d'une flore arbustive sauvage et la sauvegarde d'emplois dans des zones menacées par la désertification des campagnes.

Rare Breeds International (RBI) is the global non-governmental organization (NGO) concerned with the conservation of animal genetic resources (AnGR). It works in conjunction with FAO and with other organizations associated with livestock production. RBI members form a grassroots network that is rich in experience and knowledge of native breeds and affiliated national organizations maintain a valuable database of AnGR. Information is disseminated through major international conferences which are held at intervals of three years and through annual regional meetings. The cumulative expertise of the RBI membership facilitates a wide variety of projects, which range from policy interaction with governmental agencies to emergency rescue action and breeding programmes for relic breeds. RBI communicates mainly through publication of proceedings of major conferences and also by dedicated and linked websites.