Mesotrione, a carotenoid biosynthesis inhibitor, is being evaluated for use in turfgrass systems. It was hypothesized that root absorption of soil-applied mesotrione is necessary for effective weed control. Greenhouse studies were conducted to compare the effects of foliar-, soil-, and soil-plus-foliar–applied mesotrione at 0.14 and 0.28 kg ai/ha on yellow nutsedge and large crabgrass. In general, greatest control of yellow nutsedge and large crabgrass was by treatments that included soil application. In addition, mesotrione applied at 0.28 kg/ha generally controlled both yellow nutsedge and large crabgrass more effectively than mesotrione applied at 0.14 kg/ha. Soil- and soil-plus-foliar–applied mesotrione at 0.28 kg/ha controlled yellow nutsedge more than foliar-applied mesotrione 56 d after treatment. Soil-plus-foliar–applied mesotrione at 0.28 kg/ha controlled large crabgrass more than any other treatment 28 d after treatment. Soil- and soil-plus-foliar–applied mesotrione at both rates reduced large crabgrass foliar dry weight more effectively than did foliar-applied mesotrione. Results indicate that root absorption of mesotrione from soil is beneficial for the effective control of both yellow nutsedge and large crabgrass. For this reason, methods such as granular or high-volume applications, which enhance delivery of mesotrione to soil, would be potentially beneficial for turfgrass weed control.