This article examines the role of environmental non-governmental organizations (ENGOs) in Russia and the impact of tightening governmental accountability measures. Drawing on 18 interviews conducted in 2012–13 with Russian and international ENGOs, the article examines three key governance issues, namely: the collaborative relationship between the state and ENGOs, the impact of accountability measures on ENGO activities, and the relationships between ENGOs themselves. The findings reveal that ENGOs maintain a legitimate and effective role within Russian environmental governance. However, their legitimacy and success is significantly limited and threatened by increasing accountability measures and state actions. The article accordingly identifies a number of recommendations for increasing the likelihood of successful ENGO action in Russian environmental governance, including improving ENGO collaboration with the state and resolving tensions between participation and accountability.