The study the diet of Eneolithic populations is of great interest to archaeologists. However, the studies undertaken in the steppe and forest-steppe zones of Volga region in Russia have left many issues unsolved. Data collected recently through the comprehensive studies of Lebyazhinka VI settlement enable us to change this situation. Of particular importance at this settlement site is good preservation of animal bones, bone fishing tools, and ceramics of the same type with food crusts and connected to a large house pit. For the first time in this geographical area, bones of domestic animals were found in the fill of a dwelling. The aim of this paper is to present the results obtained through comprehensive studies of diet and economy in the Eneolithic based on the materials from Lebyazhinka III and Lebyazhinka VI settlement sites. The main results of the archaeozoological analysis— determinations of species, age and size of the animals—provide the necessary data for studying the diet. We conclude that there are differences between Lebyazhinka III and Lebyazhinka VI settlements. Lebyazhinka III settlement included bones of only wild species, however, Lebyazhinka VI settlement consists of wild and domestic species.