Okenia aspersa is a north-eastern Atlantic species, only previously recorded as Okenia quadricornis from the Malta Archipelago in the Mediterranean Sea, and in juvenile forms, from Sicily and the Gulf of Naples. A second adult specimen is recorded in the present paper, which was found along the Noli Cape coast, in the western Ligurian Sea (Italy). It was found on pebbles at a depth of 10 m. In the same area, at a depth of 5.5 m, two specimens of Doto koenneckeri and three of their egg masses were found. This species, also described from Norway and Ireland, was only previously known to occur in the Mediterranean Sea off Spain and France. It is likely that the cold winter temperatures of the Ligurian Sea allowed this species to live and reproduce.