Simple active radiometer sensors, such as RapidScan, enable agronomic decision-making and phenotyping within commercial wheat fields and experiments. The objectives of this study were: 1 - to evaluate the accuracy of quantitative biomass and nitrogen uptake estimation by the RapidScan, and 2 - to evaluate yield loss estimation based on NDVI. The RapidScan sensor was used as an assessment tool in the following studies: (i) over 3 years, 518 wheat samples were monitored during the vegetative growth period for biomass and aboveground nitrogen uptake and (ii) wheat cultivars were tested in an additional 4 field experiments, which were scanned weekly and correlated with grain yield. Results showed that accurate biomass estimation is limited up to about 100 g DM m−2. Grain yield, actual and potential, estimation is highly affected by the emergence date. The results showed that the use of a proximal-sensing technique allows for rapid and accurate crop monitoring and yield estimation, but emphasizing limitations in future use as well.