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1 results

  • Construction of Ipr1 expression vector and development of cloned embryos in vitro

  • Yongli Song, Xiaoning He, Song Hua, Jie Lan, Yonggang Liu, Pang Cheng, Hailin Zhang, Jixia Li, Xiaoying He, Jun Liu, Yong Zhang
  • Journal:
    Zygote / Volume 21 / Issue 3 / August 2013
    Published online by Cambridge University Press:
    26 August 2011, pp. 265-269

  • The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP–EGFP–Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals.