The weed sicklepod, a problem in the cultivation of soybean, cotton, and peanut, can be controlled by the mycoherbicide, Alternaria cassiae. However, the presence of water (dew) for an extended period is required by the fungal spores to germinate and infect the target weed. Preliminary tests with invert (water-in-oil) emulsions containing paraffin wax, paraffin oil, lecithin, and water indicated that about 10% (w/w) water, maintained over an 18-h period, was necessary under laboratory conditions for significant germination of the fungus. The ability to maintain that amount of water in the invert emulsion depended upon spray deposit size, adjuvants, and oil phase: water ratio. Paraffin wax was an effective adjuvant for reducing water evaporation and increasing sicklepod mortality in the greenhouse.

The interactions of two fungal biocontrol agents, Alternaria cassiae and Pseudocercospora nigricans, and soybean planting density on sicklepod mortality and dry weight were studied in the field over 2 yr. The experimental field was divided into three equal areas: one without soybean and two where the soybean was sown in densities of 20 and 36 seeds per meter row with a 0.95-m row spacing. The fungi were sprayed alone or in a mixture at three growth stages of sicklepod plants grown at three levels of crop interference resulting from the three soybean planting densities. The fungal treatments were: an untreated control, A. cassiae (105 spores/m2), P. nigricans (3.3 g mycelium/m2), and the mixture of these two fungi. Sicklepod was at the cotyledonary leaf, two-leaf, and four-leaf stages when treated. Alternaria cassiae was most effective in reducing both sicklepod survival and dry weight. The mixture of P. nigricans and A. cassiae was generally comparable to but not better than A. cassiae alone in killing the weed (mortality) and reducing its growth (dry weight). Soybean density did not have significant effects on the mortality or the dry weight of sicklepod. Thus, there is no advantage to combining the highly effective biocontrol agent A. cassiae with the less effective P. nigricans or with soybean interference to control sicklepod. However, the results validate the efficacy of A. cassiae by itself as a bioherbicide.

An invert emulsion with low viscosity and excellent water-holding properties was developed for delivery of fungal weed pathogens (mycoherbicides). Alternaria cassiae, a pathogen of sicklepod, proliferated after incorporation of spores in the emulsion. The oil phase (CDQ-1) contained paraffin wax, a paraffinic spray oil, and an unsaturated monoglyceride emulsifier (Myverol 18–99). The oil phase was mixed 1:1 (by weight) with water to form the invert, which was sprayed onto glass plates for testing of water retention properties. Water contents ranged from 22% (with A. cassiae present) to 41% (without fungus) for 0.6 to 0.7-mm2 spray deposits after 24-h storage at 21 C and 65% relative humidity. An invert emulsion containing lactofen was prepared using the CDQ-1 formulation.