A One Health approach that goes beyond human health has the potential to provide global health security (including prevention of future pandemics and reduction in antimicrobial resistant infections), provide food safety and security, as well as general environmental security. To answer the challenges to implementing such an all-embracing One Health approach, Wildlife Health Australia has grown from a primarily veterinarian-based organisation to an organisation working with governments, academia, and civil society across biosecurity to environment issues. Wildlife Health Australia sees its role in helping Australia, and the wider Indo-Pacific region, adopt a radical change in thinking to position people, animal, plant, and ecosystem health as mutual beneficiaries from societies’ investments and interventions. For the future, Wildlife Health Australia has adopted a Theory of Change leading to an impact mirroring the Convention on Biological Diversity’s 2050 Vision: “People living in harmony with nature.”