Monoclonal antibodies were raised against the mycelium of a
litter-decomposing basidiomycete, Mycena galopus, for use in an
autecological study of the fungus in forest litter. Two clones
(3E12 and 6D8), produced using particulate and whole-cell extracts of
the fungus respectively, cross-reacted with several species including
other Mycena species, non-Mycena basidiomycetes, zygomycetes
and anamorphs or mitosporic fungi. Of these clones, 3E12 appeared to
be directed against an unidentified β-glucan and 6D8 against
chitin. A third clone (6E1), using as immunogen latex collected in the
field from stipes of the basidiomes, was specific to M. galopus
mycelium in ELISA and immunofluorescence tests and was probably directed
against an unidentified polysaccharide.