Of three hundred and sixty Trichoderma strains investigated, fourteen, identified as T. aureoviride, T. harzianum and T. viride, grew
well at 5 °C on both minimal and yeast extract agar media. In dual culture tests at 10°, most strains antagonized the
phytopathogens Rhizoctonia solani and Fusarium oxysporum f. sp. dianthi. T. aureoviride and T. viride were more effective against
Pythium debaryanum than T. harzianum. The activities of extracellular chitinases (EC 3.2.1.30), β-glucosidases (EC 3.2.1.21) and
proteases (EC 3.4.21.1; EC 3.4.21.4), which are thought to be involved in the mycoparasitic process, were also examined and
results showed that these enzymes were highly active at 5° in the cold-tolerant strains.