Previous results have shown in AIDS children diminished levels of sera selenium(Reference Pallaro, Barbeito and Strasnoy1). Due to the antioxidant effect of this mineral, its deficiency could deteriorate the number of immune cells and/or their function. The aim of this preliminary study is to evaluate selenium status and total antioxidant capacity in a group of AIDS adults. Twenty two patients AIDS between 25 and 50 years old were included. Samples of whole blood were collected from fasting patients. Sera selenium (Se) was determined in haemolysis-free plasma by flame atomic absorption spectrometry; a calibration curve was performed, using commercial standards. Reference values were taken from international bibliography (60–160 μg/l)(2–Reference Lockitch, Halstead and Wadsworth4). Total antioxidant capacity (TAC) was determined as described by Miller et al. and Re et al.(Reference Miller, Rice-Evans and Davies5, Reference Re, Pellegrini and Proteggente6) and was compared with those obtained on a healthy adult group (mean=1.92, sd=0.04). The Ethics Committee of the University of Buenos Aires approved the study. All participants gave informed consent before recruitment. Statistical analyses were performed with the Student's t test.
The results expressed as means (sd) were: Se (μg/l) 34.7 (16.7) and TAC (mM – Trolox equivalents(Reference Re, Pellegrini and Proteggente6)) 1.69 (0.18)(Reference Miller, Rice-Evans and Davies5, Reference Re, Pellegrini and Proteggente6). When the results were compared with reference values, diminution in their levels were observed (P<0.01). It is important to point out that 81.8% of the patients presented selenium concentration less than 60 μg/l. The same behaviour was observed in other AIDS populations(Reference Pallaro, Barbeito and Strasnoy1, Reference Stambullian, Feliu and López7). A controlled Se supplementation would be necessary to protect immune cells.
This work was supported by University of Buenos Aires (Grant No. B-074).
