Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-03T05:29:17.934Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparative aspects of in vitro proliferation of human and porcine intestinal cells exposed to mycotoxin zearalenone and its derivatives

Published online by Cambridge University Press:  19 April 2013

I. Taranu ,
D. E. Marin ,
R. Burlacu  and
G. L. Calin
I. Taranu
Affiliation:
INCDBNA, National Institute of Research and Development for Biology and Animal Nutrition, Balotesti, Romania
D. E. Marin
Affiliation:
INCDBNA, National Institute of Research and Development for Biology and Animal Nutrition, Balotesti, Romania
R. Burlacu
Affiliation:
INCDBNA, National Institute of Research and Development for Biology and Animal Nutrition, Balotesti, Romania
G. L. Calin
Affiliation:
INCDBNA, National Institute of Research and Development for Biology and Animal Nutrition, Balotesti, Romania
Rights & Permissions [Opens in a new window]

Abstract

An abstract is not available for this content. As you have access to this content, full HTML content is provided on this page. A PDF of this content is also available in through the ‘Save PDF’ action button.
Type
Abstract
Information
Proceedings of the Nutrition Society , Volume 72 , Issue OCE1: 6th International Workshop on Immunonutrition, 15th–17th October 2012, Palma de Mallorca , 2013 , E46
Copyright
Copyright © The Authors 2013 

Gastrointestinal epithelium provides a barrier against the mucosal penetration of feed and food contaminants and pathogens. In this in vitro study, we investigated and compared the cytotoxic effect of zearalenone (ZEN), mycotoxin that commonly contaminates cereals, and some of its derivatives: α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), and zearalanone (ZAN) on three different intestinal cell lines. Intestinal cell lines from human (Caco-2 and Colo 320) or porcine (IPEC-1) origin, were cultured with increasing concentrations of ZEN, α-ZOL, α-ZOL and ZAN for 48 hours and cell cytotoxicity was measured by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. The dose response curves were fitted to a non-linear regression equations and inhibiting concentration of 50% (IC50) were defined by the regression procedure using Corel QUATTRO ProX3, Mathcad Professional 14. For each mycotoxin multiple linear regressions (Corel QUATTRO Pro X3, Mathcad Professional 14) were used to establish correlations between human cells as dependent variable (y or z) and porcine intestinal cells as independent variable (x).

The results showed that increased concentrations of ZEN, α-ZOL, β-ZOL and ZAN decreased in vitro the cell viability in both species and cell types in a dose dependent manner, porcine IPEC cells being the most sensitive. The amounts of toxins necessary to inhibit viability, in a dehydrogenase enzyme activity assay, by 50% were 113.0 (ZEN), 209.2 (α-ZOL), 140.5 (β-ZOL) and 81.6 μM (ZAN) respectively in Caco-2 cells, 64.3 (ZEN), 105.9 (α-ZOL), 38.2 (β-ZOL) and 51.4 μM (ZAN) in IPEC cells and 99.95 (ZEN), 142.4 (α-ZOL), 150.5 (β-ZOL) and 215.4 μM (ZAN) in Colo-320 cells.

Based on the results of this in vitro study, high mathematically correlations coefficients (r between 0.939 and 0.990) were found between human (Caco-2) and pig (IPEC-1) intestinal cells for ZEN and its derivatives which suggest that pig could be used as a suitable model for comparison and for extrapolation to the human situations the effects concerning these mycotoxins.