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Methylation patterns in 5′ terminal regions of pluripotency-related genes in mature bovine gametes

Published online by Cambridge University Press:  07 July 2010

Jie Lan ,
Song Hua ,
Yuan Yuan ,
Liping Zhan ,
Xiaoning He  and
Yong Zhang
Jie Lan
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Song Hua
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Yuan Yuan
Affiliation:
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Liping Zhan
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Xiaoning He
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Yong Zhang*
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
*
All correspondence to: Yong Zhang. Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of Biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China. Tel: +86 29 87080092. Fax: +86 29 87080085. e-mail: [email protected]
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Summary

Gametogenesis is associated with DNA methylation and involves complicated and delicate gene regulation network in which stem cell marker genes exert their functions. Therefore, it is necessary to investigate DNA methylation profiles of those genes in mature gametes that have an effect on embryo development. However, to date, there are limited data available on these genes in mature gametes of bovine. Here we show methylation profiles in 5′ terminal regions of five pluripotency-related genes (Oct4, Sox2, Nanog, Rex1 and Fgf4) in bovine mature gametes, based on the reasoning that the five genes harbour CpG islands in their own 5′ terminal regions, which are frequently the targets of DNA methylation. The results showed that Oct4 and Fgf4 exhibited significant hypermethylation in sperm compared with that in oocytes (p < 0.01), while Sox2 and Nanog displayed relatively the same methylation levels between sperm and oocytes (p > 0.05). Additionally, Rex1 showed a relatively high methylation level in sperm than in oocytes, although no significant differences were found (p > 0.05). In conclusion, bovine mature gametes exhibited two methylation profiles in terms of the five genes, one being non-sex-specific and the other being sex-specific.

Keywords

BovineDNA methylationOocytePluripotency-related genesSperm
Type
Research Article
Information
Zygote , Volume 19 , Issue 2 , May 2011 , pp. 165 - 169
Copyright
Copyright © Cambridge University Press 2010

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References

Benchaib, M., Braun, V., Ressnikof, D., Lornage, J., Durand, P., Niveleau, A. & Guerin, J.F. (2005). Influence of global sperm DNA methylation on IVF results. Hum. Reprod. 20, 768–73.CrossRefGoogle ScholarPubMed
Chambers, I., Silva, J., Colby, D., Nichols, J., Nijmeijer, B., Robertson, M., Vrana, J., Jones, K., Grotewold, L. & Smith, A. (2007). Nanog safeguards pluripotency and mediates germline development. Nature 450, U1230–8.CrossRefGoogle ScholarPubMed
Dean, W., Santos, F. & Reik, W. (2003). Epigenetic reprogramming in early mammalian development and following somatic nuclear transfer. Semin. Cell Dev. Biol. 14, 93–100.CrossRefGoogle ScholarPubMed
Geijsen, N. & Jones, D.L. (2008). Seminal discoveries in regenerative medicine: contributions of the male germ line to understanding pluripotency. Hum. Mol. Genet. 17, R16–22.CrossRefGoogle ScholarPubMed
Hajkova, P., Erhardt, S., Lane, N., Haaf, T., El-Maarri, O., Reik, W., Walter, J. & Surani, M. A. (2002). Epigenetic reprogramming in mouse primordial germ cells. Mech. Dev. 117, 15–23.CrossRefGoogle ScholarPubMed
Hammoud, S.S., Nix, D.A., Zhang, H., Purwar, J., Carrell, D.T., Cairns, B.R. (2009). Distinctive chromatin in human sperm packages genes for embryo development. Nature 460, 473–8.CrossRefGoogle ScholarPubMed
Hua, S., Zhang, Y., Song, K., Song, J.M., Zhang, Z.P., Zhang, L., Zhang, C., Cao, J.W. & Ma, L.B. (2008). Development of bovine–ovine interspecies cloned embryos and mitochondria segregation in blastomeres during preimplantation. Anim. Reprod. Sci. 105, 245–57.CrossRefGoogle ScholarPubMed
Kafri, T., Ariel, M., Brandeis, M., Shemer, R., Urven, L., McCarrey, J., Cedar, H. & Razin, A. (1992). Developmental pattern of gene-specific DNA methylation in the mouse embryo and germline. Genes Dev. 6, 705–14.CrossRefGoogle Scholar
Kristensen, D.M., Nielsen, J.E., Skakkebaek, N.E., Graem, N., Jacobsen, G.K., Meyts, E.R.D. & Leffers, H. (2008). Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms. Hum. Reprod. 23, 775–82.CrossRefGoogle ScholarPubMed
Lin, L., Li, Q., Zhang, L., Zhao, D.S., Dai, Y.P. & Li, N. (2008). Aberrant epigenetic changes and gene expression in cloned cattle dying around birth. BMC Dev. Biol. 8, 14.CrossRefGoogle ScholarPubMed
Patra, S.K., Patra, A., Rizzi, F., Ghosh, T.C. & Bettuzzi, S. (2008). Demethylation of (Cytosine-5-C-methyl). DNA and regulation of transcription in the epigenetic pathways of cancer development. Cancer Metastasis Rev. 27, 315–34.CrossRefGoogle ScholarPubMed
Pesce, M., Wang, X.Y., Wolgemuth, D.J. & Scholer, H. (1998). Differential expression of the Oct-4 transcription factor during mouse germ cell differentiation. Mech. Dev. 71, 89–98.CrossRefGoogle ScholarPubMed
Reik, W., Santos, F. & Dean, W. (2003). Mammalian epigenomics: reprogramming the genome for development and therapy. Theriogenology 59, 21–32.CrossRefGoogle Scholar
Rousseaux, S., Reynoird, N., Escoffier, E., Thevenon, J., Caron, C. & Khochbin, S. (2008). Epigenetic reprogramming of the male genome during gametogenesis and in the zygote. Reprod. Biomed. Online 16, 492–503.CrossRefGoogle ScholarPubMed
Swales, A.K.E. & Spears, N. (2005). Genomic imprinting and reproduction. Reproduction 130, 389–99.CrossRefGoogle ScholarPubMed
Wrenzycki, C., Herrmann, D., Keskintepe, L., Martins, A., Sirisathien, S., Brackett, B. & Niemann, H. (2001). Effects of culture system and protein supplementation on mRNA expression in preimplantation bovine embryos. Hum. Reprod. 16, 893–901.CrossRefGoogle Scholar